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Literature summary for 4.2.3.25 extracted from

  • Herrero, O.; Ramon, D.; Orejas, M.
    Engineering the Saccharomyces cerevisiae isoprenoid pathway for de novo production of aromatic monoterpenes in wine (2008), Metab. Eng., 10, 78-86.
    View publication on PubMed

Application

Application Comment Organism
biotechnology biotechnological potential of the engineered wine yeast to modify the sensorial qualities of wine Clarkia breweri

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli strain DH5alpha is used as the strain for cloning experiments and plasmid amplification. Saccharomyces cerevisiae wine strain T73-4 is used for functional expression of plasmids bearing the expression cassette. Clarkia breweri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + H2O Clarkia breweri isoprenoid pathway S-linalool + diphosphate
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?

Organism

Organism UniProt Comment Textmining
Clarkia breweri Q96376
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-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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the transformant strain with the linalool synthase encoding gene is able to produce wine in the same way as the industrial T73 strain except for the quality trait introduced. The Saccharomyces cerevisiae industrial wine strain expressing a plant monoterpene synthase has the potential to alter the monoterpene profile of wine Clarkia breweri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate + H2O isoprenoid pathway Clarkia breweri S-linalool + diphosphate
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?

Synonyms

Synonyms Comment Organism
linalool synthase
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Clarkia breweri
LIS
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Clarkia breweri
S-linalool synthase
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Clarkia breweri