Literature summary for 4.2.3.24 extracted from

Lubertozzi, D.; Keasling, J.D.
Expression of a synthetic Artemesia annua amorphadiene synthase in Aspergillus nidulans yields altered product distribution (2008), J. Ind. Microbiol. Biotechnol., 35, 1191-1198.

Application

Application	Comment	Organism
pharmacology	amorpha-4,11-diene is a precursor of artemisinin, an important agent in the treatment of malaria, produced via oxidation	Artemisia annua

Cloned(Commentary)

Cloned (Comment)	Organism
expression of a synthetic Artemesia annua amorphadiene synthase gene under control of a strong constitutive promoter ((p)gpdA) in Aspergillus nidulans yields altered product distribution. The transformants produces only small amounts of amorphadiene, but much larger amounts of similar sesquiterpenes normally produced as minor by-products in plants. Expression of ADS in Escherichia coli produces almost exclusively amorpha-4,11-diene. The host environment can greatly impact the terpenes produced from terpene synthases.	Artemisia annua

Cloned (Comment)

Organism

expression of a synthetic Artemesia annua amorphadiene synthase gene under control of a strong constitutive promoter ((p)gpdA) in Aspergillus nidulans yields altered product distribution. The transformants produces only small amounts of amorphadiene, but much larger amounts of similar sesquiterpenes normally produced as minor by-products in plants. Expression of ADS in Escherichia coli produces almost exclusively amorpha-4,11-diene. The host environment can greatly impact the terpenes produced from terpene synthases.

Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-trans,6-trans-farnesyl diphosphate	Artemisia annua	-	amorpha-4,11-diene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Artemisia annua	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Artemisia annua	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-trans,6-trans-farnesyl diphosphate	-	Artemisia annua	amorpha-4,11-diene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
ADS	-	Artemisia annua
amorpha-4,11-diene synthase	-	Artemisia annua
amorphadiene synthase	-	Artemisia annua