Cloned (Comment) | Organism |
---|---|
DNA library construction, phage display, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli as N-terminal thioredoxin fusion protein | Solidago canadensis |
General Stability | Organism |
---|---|
glycerol stabilizes the enzyme | Solidago canadensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.0, 25°C, recombinant enzyme | Solidago canadensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Solidago canadensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64700 | - |
x * 64700, about, DNA sequence calculation | Solidago canadensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | Solidago canadensis | - |
(+)-(10R)-germacrene A + diphosphate | 98% of total product is (+)-(10R)-germacrene A, 2% is alpha-humulene in feeding experiments in vivo | ? | |
additional information | Solidago canadensis | sesquiterpene hydrocarbon profile of leaf extracts, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solidago canadensis | Q9AR67 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant thioredoxin fusion enzyme from Escherichia coli by nickel chelate affinity chromatography | Solidago canadensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Solidago canadensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | - |
Solidago canadensis | (+)-(10R)-germacrene A + diphosphate | 98% of total product is (+)-(10R)-germacrene A, 2% is alpha-humulene in feeding experiments in vivo | ? | |
2-trans,6-trans-farnesyl diphosphate | - |
Solidago canadensis | (+)-(10R)-germacrene A + diphosphate | chiral GC-MS product analysis | ? | |
additional information | sesquiterpene hydrocarbon profile of leaf extracts, overview | Solidago canadensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64700, about, DNA sequence calculation | Solidago canadensis |
More | molecular modeling, structure analysis, enzyme contains the conserved motif DDXXD/E | Solidago canadensis |
Synonyms | Comment | Organism |
---|---|---|
(+)-(10R)-germacrene A synthase | - |
Solidago canadensis |
Sc1 | - |
Solidago canadensis |
sesquiterpene synthase | - |
Solidago canadensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Solidago canadensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Solidago canadensis |