Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.22 extracted from

  • Cane, D.E.; He, X.; Kobayashi, S.; Omura, S.; Ikeda, H.
    Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase (2006), J. Antibiot., 59, 471-479.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
for construction of a geoA-deletion mutant of Streptomyces avermitilis, and for expression in Escherichia coli BL21DE3 cells Streptomyces avermitilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000075
-
farnesyl diphosphate
-
Streptomyces avermitilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10-20% activity, compared to reaction with Mg2+ Streptomyces avermitilis
Cu2+ 10-20% activity, compared to reaction with Mg2+ Streptomyces avermitilis
Fe2+ 50% activity, compared to reaction with Mg2+ Streptomyces avermitilis
Mg2+ 100% activity Streptomyces avermitilis
Mn2+ 10-20% activity, compared to reaction with Mg2+ Streptomyces avermitilis
Zn2+ 50% activity, compared to reaction with Mg2+ Streptomyces avermitilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
determined by SDS-PAGE Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q82L49
-
-

Purification (Commentary)

Purification (Comment) Organism
using Q-Sepharose resin and a Q-Sepharose column Streptomyces avermitilis

Renatured (Commentary)

Renatured (Comment) Organism
resuspended inclusion body protein is refolded Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
Streptomyces avermitilis (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate 66% ?

Synonyms

Synonyms Comment Organism
germacradienol/germacrene D synthase
-
Streptomyces avermitilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0031
-
farnesyl diphosphate
-
Streptomyces avermitilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
activity assay Streptomyces avermitilis

pH Range

pH Minimum pH Maximum Comment Organism
7.6 9
-
Streptomyces avermitilis