Cloned (Comment) | Organism |
---|---|
for construction of a geoA-deletion mutant of Streptomyces avermitilis, and for expression in Escherichia coli BL21DE3 cells | Streptomyces avermitilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000075 | - |
farnesyl diphosphate | - |
Streptomyces avermitilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10-20% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
Cu2+ | 10-20% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
Fe2+ | 50% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
Mg2+ | 100% activity | Streptomyces avermitilis | |
Mn2+ | 10-20% activity, compared to reaction with Mg2+ | Streptomyces avermitilis | |
Zn2+ | 50% activity, compared to reaction with Mg2+ | Streptomyces avermitilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
determined by SDS-PAGE | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | Q82L49 | - |
- |
Purification (Comment) | Organism |
---|---|
using Q-Sepharose resin and a Q-Sepharose column | Streptomyces avermitilis |
Renatured (Comment) | Organism |
---|---|
resuspended inclusion body protein is refolded | Streptomyces avermitilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | - |
Streptomyces avermitilis | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | 66% | ? |
Synonyms | Comment | Organism |
---|---|---|
germacradienol/germacrene D synthase | - |
Streptomyces avermitilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
farnesyl diphosphate | - |
Streptomyces avermitilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
activity assay | Streptomyces avermitilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9 | - |
Streptomyces avermitilis |