Literature summary for 4.2.3.20 extracted from

Satomi, Y.; Ohara, K.; Yazaki, K.; Ito, M.; Honda, G.; Nishino, H.
Production of the monoterpene limonene and modulation of apoptosis-related proteins in embryonic-mouse NIH 3T3 fibroblast cells by introduction of the limonene synthase gene isolated from Japanese catnip (Schizonepeta tenuifolia) (2009), Biotechnol. Appl. Biochem., 52, 185-190.

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Application

Application	Comment	Organism
drug development	D-limonene is effective in cancer prevention in animal cancer models, some clinical applications have been performed	Nepeta tenuifolia

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in NIH 3T3 cell	Nepeta tenuifolia
The cDNA of D-limonene synthase obtained from Japanese catnip, modified by deletion of the transit peptide sequence and addition of a new ATG initiation codon, is cloned. Establishment of transgenic mouse embryonic NIH 3T3 fibroblast cells that produce limonene by introducing the D-limonene synthase gene. Apoptosis is not observed in the limonene-producing cells.	Nepeta tenuifolia

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranyl diphosphate	Nepeta tenuifolia	single step in plants	D-limonene + diphosphate	D-limonene shows cancer preventative and cancer therapeutic activities in vitro and in vivo, its modulates apoptosis-related genes and proteins in cultured cells	?

Organism

Organism	UniProt	Comment	Textmining
Nepeta tenuifolia	Q9FUW5	-	-
Nepeta tenuifolia	Q9FUW5	production of the monoterpene limonene and modulation of apoptosis-related proteins in embryonic-mouse NIH 3T3 fibroblast cells by introduction of the limonene synthase gene isolated from japanese catnip	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Nepeta tenuifolia	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate	single step in plants	Nepeta tenuifolia	D-limonene + diphosphate	D-limonene shows cancer preventative and cancer therapeutic activities in vitro and in vivo, its modulates apoptosis-related genes and proteins in cultured cells	?
additional information	a concomitant increase in the level of apoptosis-related protein B-cell lymphoma protein 2 and decreases in the levels of Bcl-2 antagonist of cell death and phosphorylated c-Jun N-terminal kinase are observed in limonene-producing cells	Nepeta tenuifolia	?	-	?
additional information	limonene can be synthesized in animal cells using their own mevalonate pathway and limonene-producing cells may provide a new system for the elucidation of limonene function	Nepeta tenuifolia	?	-	?

Synonyms

Synonyms	Comment	Organism
d-limonene synthase	-	Nepeta tenuifolia
limonene synthase	-	Nepeta tenuifolia

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Release 2023.1 (January 2023)