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Literature summary for 4.2.3.20 extracted from

  • Landmann, C.; Fink, B.; Festner, M.; Dregus, M.; Engel, K.H.; Schwab, W.
    Cloning and functional characterization of three terpene synthases from lavender (Lavandula angustifolia) (2007), Arch. Biochem. Biophys., 465, 417-429.
    View publication on PubMed
Search for more literature for 4.2.3.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lavandula angustifolia
into the pGEM-T vector for sequencing and into the pGEX-T-1 vector for expression in Escherichia coli cells Lavandula angustifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0474
-
 geranyl diphosphate pH 7.0, 30°C Lavandula angustifolia
0.0474
-
 geranyl diphosphate LaLIMS_RR, truncated protein Lavandula angustifolia

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Lavandula angustifolia
Mg2+ or Mn2+, reuired. Optimum concentraion 50 mM Lavandula angustifolia
Mn2+ the proportions of the products change significantly when Mn2+ is supplied instead of Mg2+ Lavandula angustifolia
Mn2+ or Mg2+, required. Optimum concentration 0.2 mM, 68% of the efficieny with Mg2+. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ Lavandula angustifolia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70300
-
 x * 70300, SDS-PAGE Lavandula angustifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate Lavandula angustifolia
-
 (+)-(4R)-limonene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Lavandula angustifolia Q2XSC6
-
-

Purification (Commentary)

Purification (Comment) Organism
using GST bind resin Lavandula angustifolia

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Lavandula angustifolia
-
leaf
-
 Lavandula angustifolia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
 Lavandula angustifolia (+)-(4R)-limonene + diphosphate
-
 ?
geranyl diphosphate
-
 Lavandula angustifolia (1R,5R)-(+)-alpha-pinene + diphosphate
-
 ?
geranyl diphosphate
-
 Lavandula angustifolia (1R,4S)-(+)-camphene + diphosphate
-
 ?
geranyl diphosphate
-
 Lavandula angustifolia alpha-phellandrene + diphosphate
-
 ?
geranyl diphosphate
-
 Lavandula angustifolia beta-myrcene + diphosphate
-
 ?
geranyl diphosphate
-
 Lavandula angustifolia terpinolene + diphosphate
-
 ?
geranyl diphosphate
-
 Lavandula angustifolia (R)-limonene + diphosphate products are 39% (R)-limonene, 22% terpinolene, 16% (1R,5S)-camphene, 14% (1R,5R)-(+)-alpha-pinene, 8% betamyrcene and traces of alpha-phellandrene. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ ?
additional information no substrates: farnesyl diphosphate, geranylgeranyl diphosphate Lavandula angustifolia ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70300, SDS-PAGE Lavandula angustifolia

Synonyms

Synonyms Comment Organism
(R)-limonene synthase
-
 Lavandula angustifolia
LaLIMS
-
 Lavandula angustifolia
limonene synthase
-
 Lavandula angustifolia
LIMS
-
 Lavandula angustifolia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 standard activity assay Lavandula angustifolia
30
-
-
 Lavandula angustifolia
30
-
 optimized assay, LaLIMS_RR, truncated protein Lavandula angustifolia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 geranyl diphosphate LaLIMS_RR, truncated protein Lavandula angustifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Lavandula angustifolia
7
-
 optimized assay, LaLIMS_RR, truncated protein Lavandula angustifolia
7.5
-
 standard activity assay Lavandula angustifolia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.255
-
 geranyl diphosphate pH 7.0, 30°C Lavandula angustifolia