Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.199 extracted from

  • Fang, X.; Li, C.Y.; Yang, Y.; Cui, M.Y.; Chen, X.Y.; Yang, L.
    Identification of a novel (-)-5-epieremophilene synthase from Salvia miltiorrhiza via transcriptome mining (2017), Front. Plant Sci., 8, 627 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.199

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta (DE3) Salvia miltiorrhiza
transient expression in Nicotiana benthamiana Salvia miltiorrhiza

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01044
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS3 Salvia miltiorrhiza
0.0242
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS2 Salvia miltiorrhiza
0.02535
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS1 Salvia miltiorrhiza

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ metal cofactor Salvia miltiorrhiza

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl-diphosphate Salvia miltiorrhiza
-
 (-)-5-epieremophilene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Salvia miltiorrhiza A0A1W6GW06
-
-
Salvia miltiorrhiza A0A1W6GW18
-
-
Salvia miltiorrhiza A0A1W6GW32
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salvia miltiorrhiza

Source Tissue

Source Tissue Comment Organism Textmining
inflorescence mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root Salvia miltiorrhiza
-
inflorescence mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed Salvia miltiorrhiza
-
inflorescence mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS3 is expressed predominantly in inflorescence Salvia miltiorrhiza
-
leaf mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root Salvia miltiorrhiza
-
leaf mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS1 is expressed at a higher level than the SmSTPS2 and SmSTPS3, and its transcripts are most abundant in leaf Salvia miltiorrhiza
-
leaf mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed and exhibits a clear expression only in leaf Salvia miltiorrhiza
-
root mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root Salvia miltiorrhiza
-
root mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed Salvia miltiorrhiza
-
stem mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root Salvia miltiorrhiza
-
stem mainly expressed in leaf and inflorescence, transcript level is low or undetectable in stem and root. SmSTPS2 is weakly expressed Salvia miltiorrhiza
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl-diphosphate
-
 Salvia miltiorrhiza (-)-5-epieremophilene + diphosphate
-
 ?
(2E,6E)-farnesyl-diphosphate (-)-5-epieremophilene i.e. (3R,4aS,5S)-4a,5-dimethyl-3-(prop-1-en-2-yl)-1,2,3,4,4a,5,6,7-octahydronaphthalene. Salvia miltiorrhiza produces three different forms of the enzyme, encoded by paralogous genes, that exhibit different spacial expression patterns and respond differently to hormone treatment (STPS1, STP2m and STP3) Salvia miltiorrhiza (-)-5-epieremophilene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
STP2
-
 Salvia miltiorrhiza
STP3
-
 Salvia miltiorrhiza
STPS1
-
 Salvia miltiorrhiza

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Salvia miltiorrhiza

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.53
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS3 Salvia miltiorrhiza
2.07
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS2 Salvia miltiorrhiza
2.09
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS1 Salvia miltiorrhiza

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Salvia miltiorrhiza

Expression

Organism Comment Expression
Salvia miltiorrhiza SmSTPS1 is induced strongly by ethylene and moderately by methyl jasmonate, abscicic acid and gibberellin A3 up
Salvia miltiorrhiza SmSTPS2 is induced by ethylene or by abscicic acid up
Salvia miltiorrhiza SmSTPS3 is up-regulated slightly only by abscicic acid up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
82.7
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS1 Salvia miltiorrhiza
85.6
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS2 Salvia miltiorrhiza
148
-
 (2E,6E)-farnesyl-diphosphate 30°C, pH 7.0, enzyme form SmSTPS3 Salvia miltiorrhiza