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Literature summary for 4.2.3.19 extracted from

  • Shimane, M.; Ueno, Y.; Morisaki, K.; Oogami, S.; Natsume, M.; Hayashi, K.I.; Nozaki, H.; Kawaide, H.
    Molecular evolution of the substrate specificity of ent-kaurene synthases to adapt to gibberellin biosynthesis in land plants (2014), Biochem. J., 462, 539-546.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15(DE3) cells Selaginella moellendorffii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
copalyl diphosphate Selaginella moellendorffii
-
sandaracopimaradiene + diphosphate
-
?
ent-copalyl diphosphate Selaginella moellendorffii
-
ent-kaurene + diphosphate
-
?
additional information Selaginella moellendorffii the enzyme prefers normal and syn-copalyl diphosphate over ent-copalyl diphosphate ?
-
?
syn-copalyl diphosphate Selaginella moellendorffii
-
?
-
?

Organism

Organism UniProt Comment Textmining
Selaginella moellendorffii A0A077JGG8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Selaginella moellendorffii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
copalyl diphosphate
-
Selaginella moellendorffii sandaracopimaradiene + diphosphate
-
?
ent-copalyl diphosphate
-
Selaginella moellendorffii ent-kaurene + diphosphate
-
?
additional information the enzyme prefers normal and syn-copalyl diphosphate over ent-copalyl diphosphate Selaginella moellendorffii ?
-
?
syn-copalyl diphosphate
-
Selaginella moellendorffii ?
-
?

Synonyms

Synonyms Comment Organism
CPS/KS
-
Selaginella moellendorffii
ent-kaurene synthase
-
Selaginella moellendorffii