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Literature summary for 4.2.3.19 extracted from

  • Hayashi, K.; Kawaide, H.; Notomi, M.; Sakigi, Y.; Matsuo, A.; Nozaki, H.
    Identification and functional analysis of bifunctional ent-kaurene synthase from the moss Physcomitrella patens (2006), FEBS Lett., 580, 6175-6181.
    View publication on PubMed
Search for more literature for 4.2.3.19

Cloned(Commentary)

Cloned (Comment) Organism
into the pTD1 vector as a fusion of copalyl diphosphate synthase and ent-kaurene synthase for in vitro expression Physcomitrium patens

Protein Variants

Protein Variants Comment Organism
D635A/D636A mutation of the DDYFD motif in the kaurene synthase domain Physcomitrium patens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
 copalyl diphosphate synthase-kaurene synthase fusion protein, determined by SDS-PAGE and Western blot analysis Physcomitrium patens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Physcomitrium patens
-
 ent-kaurene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Physcomitrium patens A5A8G0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Physcomitrium patens ent-kaurene + diphosphate
-
 ?
geranylgeranyl diphosphate PpCPS-KS directly synthesizes the ent-kaurene skeleton from GGDP Physcomitrium patens ent-kaurene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ent-kaurene synthase
-
 Physcomitrium patens
KS
-
 Physcomitrium patens
PpCPS/KS bifunctional cyclase with copalyl disphosphatase and kaurene synthase activity Physcomitrium patens
PpKS
-
 Physcomitrium patens