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Literature summary for 4.2.3.19 extracted from

  • Saito, T.; Abe, H.; Yamane, H.; Sakurai, A.; Murofushi, N.; Takio, K.; Takahashi, N.; Kamiya, Y.
    Purification and properties of ent-kaurene synthase B from immature seeds of pumpkin (1995), Plant Physiol., 109, 1239-1245.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.19

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Cucurbita maxima
Ca2+
-
 Cucurbita maxima
Cu2+
-
 Cucurbita maxima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 copalyl diphosphate
-
 Cucurbita maxima

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ Cucurbita maxima
Mg2+ required Cucurbita maxima
Mn2+ can partially replace Mg2+ Cucurbita maxima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 SDS-PAGE, purified enzyme Cucurbita maxima
90000
-
 gel filtration Cucurbita maxima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Cucurbita maxima involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Cucurbita maxima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
separation of activities A and B achieved by hydrophobic interaction chromatography Cucurbita maxima

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Cucurbita maxima
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 purified enzyme Cucurbita maxima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
copalyl diphosphate
-
 Cucurbita maxima sandaracopimaradiene + diphosphate
-
 ?
ent-copalyl diphosphate
-
 Cucurbita maxima ent-kaurene + diphosphate
-
 ?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Cucurbita maxima ent-kaurene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Cucurbita maxima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.5
-
 Cucurbita maxima