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Literature summary for 4.2.3.189 extracted from

  • Zerbe, P.; Chiang, A.; Dullat, H.; ONeil-Johnson, M.; Starks, C.; Hamberger, B.; Bohlmann, J.
    Diterpene synthases of the biosynthetic system of medicinally active diterpenoids in Marrubium vulgare (2014), Plant J., 79, 914-927 .
    View publication on PubMed
Search for more literature for 4.2.3.189

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Marrubium vulgare

Crystallization (Commentary)

Crystallization (Comment) Organism
ELS belongs to a subgroup of the diTPS TPS–e/f clade with unusual betaalpha-domain architecture Marrubium vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Marrubium vulgare ELS is active in vitro and in vivo with three different prenyl diphosphate substrates forming the marrubiin precursor 9,13-epoxy-labd-14-ene, manoyl oxide, i.e. reaction of EC 4.2.3.190, and miltiradiene, reaction of EC 4.2.3.131 ?
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 ?
peregrinol diphosphate Marrubium vulgare
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 (13epsilon)-9,13-epoxylabda-14-ene + diphosphate
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 ?

Organism

Organism UniProt Comment Textmining
Marrubium vulgare A0A075FBG7
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-

Source Tissue

Source Tissue Comment Organism Textmining
flower transcript level is 4fold higher in leaf compared with flower Marrubium vulgare
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leaf transcript level is 4fold higher in leaf compared with flower Marrubium vulgare
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ELS is active in vitro and in vivo with three different prenyl diphosphate substrates forming the marrubiin precursor 9,13-epoxy-labd-14-ene, manoyl oxide, i.e. reaction of EC 4.2.3.190, and miltiradiene, reaction of EC 4.2.3.131 Marrubium vulgare ?
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 ?
peregrinol diphosphate
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 Marrubium vulgare (13epsilon)-9,13-epoxylabda-14-ene + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
ELS
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 Marrubium vulgare

General Information

General Information Comment Organism
physiological function in vitro, in combination with the copalyl diphosphate-producing CPS3, ELS forms miltiradiene. When coupled with TPS2, ELS converts (13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate into manoyl oxide. The coupled reaction of CPS1 and ELS leads to 80% (13epsilon)-9,13-epoxylabda-14-ene Marrubium vulgare