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Literature summary for 4.2.3.180 extracted from

  • Mafu, S.; Karunanithi, P.S.; Palazzo, T.A.; Harrod, B.L.; Rodriguez, S.M.; Mollhoff, I.N.; OBrien, T.E.; Tong, S.; Fiehn, O.; Tantillo, D.J.; Bohlmann, J.; Zerbe, P.
    Biosynthesis of the microtubule-destabilizing diterpene pseudolaric acid B from golden larch involves an unusual diterpene synthase (2017), Proc. Natl. Acad. Sci. USA, 114, 974-979 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.180

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and transient Agrobacterium-mediated expression in Nicotiana benthamiana Pseudolarix amabilis

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling and docking of substrate. The Mg2+-coordinating DDxxD motif and arginine residues R558, R560, and R736 critical for catalysis in Abies grandis are conserved in TPS8 Pseudolarix amabilis

Protein Variants

Protein Variants Comment Organism
R558A complete loss of activity Pseudolarix amabilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Pseudolarix amabilis
-
 pseudolaratriene + diphosphate
-
 ?
additional information Pseudolarix amabilis no product is formed with geranyl diphosphate or farnesyl diphosphate or in assays with class II diterpene synthases forming copalyl diphosphate of ent- or (+)-stereochemistry ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudolarix amabilis A0A1L6Z3A0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bark expression predominantly in roots and in bark Pseudolarix amabilis
-
needle expression near the limit of detection in needle tissue Pseudolarix amabilis
-
root expression predominantly in roots and in bark Pseudolarix amabilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Pseudolarix amabilis pseudolaratriene + diphosphate
-
 ?
additional information no product is formed with geranyl diphosphate or farnesyl diphosphate or in assays with class II diterpene synthases forming copalyl diphosphate of ent- or (+)-stereochemistry Pseudolarix amabilis ?
-
 ?

Synonyms

Synonyms Comment Organism
pseudolarene synthase
-
 Pseudolarix amabilis
TPS8
-
 Pseudolarix amabilis

General Information

General Information Comment Organism
physiological function enzyme catalyzes the first committed step in pseudolaric acid B biosynthesis, showing a distinct carbocation-driven reaction mechanism en route to the 5,7-trans-fused bicyclic pseudolaratriene scaffold Pseudolarix amabilis