Literature summary for 4.2.3.18 extracted from

Zhou, K.; Gao, Y.; Hoy, J.A.; Mann, F.M.; Honzatko, R.B.; Peters, R.J.
Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis (2012), J. Biol. Chem., 287, 6840-6850.

Search for more literature for 4.2.3.18

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli C41 OverExpress and B834(DE3) cells	Abies grandis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 24% (v/v) PEG 8000, 0.1 M sodium citrate, pH 5.1, 0.1 M dibasic ammonium phosphate	Abies grandis

Protein Variants

Protein Variants	Comment	Organism
N451A	this mutation reduces class II (protonation-initiated cyclization) activity about 100fold, with essentially no effect on class I (ionization-initiated cyclization) activity	Abies grandis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(+)-copalyl diphosphate	Abies grandis	-	abieta-7,13-diene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Abies grandis	Q38710	-	-

Purification (Commentary)

Purification (Comment)	Organism
Mono Q column chromatography and type II ceramic hydroxyapatite column chromatography	Abies grandis

Storage Stability

Storage Stability	Organism
-80°C, purified enzyme in 10 mM Bis-Tris, pH 6.8, 10% (v/v) glycerol,150 mM KCl, 10 mM MgCl2, and 5 mM dithiohtreitol, several months, without significant loss of activity	Abies grandis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-copalyl diphosphate	-	Abies grandis	abieta-7,13-diene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
abietadiene synthase	-	Abies grandis

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Release 2023.1 (January 2023)