Cloned (Comment) | Organism |
---|---|
expression in Aspergillus oryzae and Escherichia coli | Aspergillus stellatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the DDXXD motif for binding a trinuclear Mg2+ cluster is conserved in both the N-terminal terpene cyclase and C-terminal prenyl tranferase domains as 92DDVTD96 and 473DDVED477, respectively. Assay in presence of Mg2+ | Aspergillus stellatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E,10E,14E)-geranylfarnesyl diphosphate | Aspergillus stellatus | - |
stellata-2,6,19-triene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus stellatus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E,10E,14E)-geranylfarnesyl diphosphate | - |
Aspergillus stellatus | stellata-2,6,19-triene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sesterterpene synthase | - |
Aspergillus stellatus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme synthesizes the C25 linear substrate (2E,6E,10E,14E)-geranylfarnesyl diphosphate from dimethylallyl diphosphate and 4 molecules of isopentenyl dipohosphate and also cyclizes (2E,6E,10E,14E)-geranylfarnesyl diphosphate into stellata-2,6,19-triene, which is further transformed into stellatic acid by a cytochrome P450 monooxygenase encoded by the gene adjacent to the sesterterpene synthase gene | Aspergillus stellatus |