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Literature summary for 4.2.3.17 extracted from

  • Soliman, S.; Tang, Y.
    Natural and engineered production of taxadiene with taxadiene synthase (2015), Biotechnol. Bioeng., 112, 229-235 .
    View publication on PubMed
Search for more literature for 4.2.3.17

Application

Application Comment Organism
synthesis taxadiene synthase ist he rate-limiting enzyme in the biosynthesis of the anticancer compound paclitaxel Taxus brevifolia

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Taxus brevifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Taxus brevifolia

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Taxus brevifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Taxus brevifolia
-
 taxa-4,11-diene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus brevifolia
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Taxus brevifolia taxa-4,11-diene + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Taxus brevifolia

Synonyms

Synonyms Comment Organism
TXS
-
 Taxus brevifolia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0106
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Taxus brevifolia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Taxus brevifolia