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Literature summary for 4.2.3.17 extracted from

  • Exposito, O.; Bonfill, M.; Onrubia, M.; Jane, A.; Moyano, E.; Cusido, R.M.; Palazon, J.; Pinol, M.T.
    Effect of taxol feeding on taxol and related taxane production in Taxus baccata suspension cultures (2008), N. Biotechnol., 25, 252-259.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
taxol exogenous Taxol (paclitaxel) causes an considerable increase in taxadiene synthase activity, the expression level peaks at day 4 with a rapid decrease at day 8, reaching a second, lower, peak at day 12. By day 16, ts mRNA is only detectable in the untreated control at a very low level. The concentration of taxol that most effectively induces ts mRNA expression is 50 mg/l compared to the control, the transcript levels are 2.5-, 3.1- and 3.3fold higher at 4, 8 and 12 days of culture. Taxus baccata

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM is included in assay medium Taxus baccata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Taxus baccata the first committed step toward taxanes and taxol biosynthesis involves taxadiene synthase which promotes the cyclization of geranylgeranyl diphosphate leading to the taxane skeleton taxa-4,11-diene + diphosphate the product of the biosynthetic step is not a limiting substrate in taxol formation ?

Organism

Organism UniProt Comment Textmining
Taxus baccata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Taxus baccata

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Taxus baccata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
a relatively high level of activity is observed at day 4, when it is 2.8-, 6.8-, 3.2- and 1.7fold higher, respectively, in the cultures treated with 25, 50, 100 and 200 mg/l taxol than in the untreated control (6.64 pkat/mg of protein). Enzyme activity then decreases markedly from days 4 to 12 and thereafter is measurable only at a very low level until day 20. Taxus baccata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate the first committed step toward taxanes and taxol biosynthesis involves taxadiene synthase which promotes the cyclization of geranylgeranyl diphosphate leading to the taxane skeleton Taxus baccata taxa-4,11-diene + diphosphate the product of the biosynthetic step is not a limiting substrate in taxol formation ?

Synonyms

Synonyms Comment Organism
taxadiene synthase
-
Taxus baccata
TXS
-
Taxus baccata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
assay at Taxus baccata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Taxus baccata