Literature summary for 4.2.3.17 extracted from

Rontein, D.; Onillon, S.; Herbette, G.; Lesot, A.; Werck-Reichhart, D.; Sallaud, C.; Tissier, A.
CYP725A4 from yew catalyzes complex structural rearrangement of taxa-4(5),11(12)-diene into the yclic ether 5(12)-oxa-3(11)-cyclotaxane (2008), J. Biol. Chem., 283, 6067-6075.

Search for more literature for 4.2.3.17

Application

Application	Comment	Organism
drug development	paclitaxel is a successful anticancer drug	Taxus cuspidata

Cloned(Commentary)

Cloned (Comment)	Organism
cDNA encoding taxa-4(5),11(12)-diene synthase is introduced in Nicotiana sylvestris for specific expression in trichome cells.	Taxus cuspidata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranylgeranyl diphosphate	Taxus cuspidata	-	taxa-4,11-diene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Taxus cuspidata	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Purification of the recombinant truncated TS protein as histag is performed using nickel-nitrilotriacetic acid affinity chromatography	Taxus cuspidata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate	-	Taxus cuspidata	taxa-4(5),11(12)-diene + diphosphate	product is biochemically synthesized using truncated TS recombinant protein from Escherichia coli, labeling reveals that 10% substrate is converted to the product	?
geranylgeranyl diphosphate	-	Taxus cuspidata	taxa-4,11-diene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
taxa-4(5),11(12)-diene synthase	-	Taxus cuspidata

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)