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Literature summary for 4.2.3.17 extracted from

  • Hezari, M.; Ketchum, R.E.B.; Gibson, D.M.; Croteau, R.
    Taxol production and taxadiene synthase activity in Taxus canadensis cell suspension cultures (1997), Arch. Biochem. Biophys., 337, 185-190.
    View publication on PubMed

Application

Application Comment Organism
medicine chemotherapeutic agent against a range of cancers, including ovarian and breast cancer Taxus brevifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
geranylgeranyl diphosphate
-
Taxus canadensis
0.025
-
Mg2+ cofactor Mg2+ Taxus canadensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Taxus canadensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
79000, SDS-PAGE Taxus canadensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Taxus brevifolia catalyzes the first step in taxol biosynthesis taxa-4,11-diene + diphosphate
-
?
geranylgeranyl diphosphate Taxus canadensis catalyzes the first step in taxol biosynthesis taxa-4,11-diene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Taxus brevifolia
-
-
-
Taxus canadensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Taxus canadensis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003
-
-
Taxus canadensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
Taxus brevifolia taxa-4(5),11(12)-diene + diphosphate
-
?
geranylgeranyl diphosphate
-
Taxus canadensis taxa-4(5),11(12)-diene + diphosphate
-
?
geranylgeranyl diphosphate catalyzes the first step in taxol biosynthesis Taxus brevifolia taxa-4,11-diene + diphosphate
-
?
geranylgeranyl diphosphate catalyzes the first step in taxol biosynthesis Taxus canadensis taxa-4,11-diene + diphosphate
-
?

Subunits

Subunits Comment Organism
? 79000, SDS-PAGE Taxus canadensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Taxus canadensis