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Literature summary for 4.2.3.163 extracted from

  • Rabe, P.; Pahirulzaman, K.A.; Dickschat, J.S.
    Structures and biosynthesis of corvol ethers-sesquiterpenes from the actinomycete Kitasatospora setae (2015), Angew. Chem. Int. Ed. Engl., 54, 6041-6045.
    View publication on PubMed
Search for more literature for 4.2.3.163

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pYE-Express, a pET28-derived plasmid that can be used for efficient cloning by homologous recombination in yeast and for heterologous expression of His-tagged recombinant enzymes in Escherichia coli Kitasatospora setae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O Kitasatospora setae
-
 (+)-corvol ether B + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + H2O Kitasatospora setae DSM 43861
-
 (+)-corvol ether B + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Kitasatospora setae E4N7E5
-
-
Kitasatospora setae DSM 43861 E4N7E5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kitasatospora setae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
 Kitasatospora setae (+)-corvol ether B + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + H2O (+)-corvol ether B i.e. (3R,5aR,6S,8aR)-3,6-dimethyl-1-(propan-2-yl)hexahydro-1H,3H-3,8a-methanocyclopenta[c]oxepine. No activity with geranylgeranyl diphosphate. The enzyme forms (+)-corvol ether B and (+)-corvol ether A in the ratio of 3:1 Kitasatospora setae (+)-corvol ether B + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + H2O
-
 Kitasatospora setae DSM 43861 (+)-corvol ether B + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + H2O (+)-corvol ether B i.e. (3R,5aR,6S,8aR)-3,6-dimethyl-1-(propan-2-yl)hexahydro-1H,3H-3,8a-methanocyclopenta[c]oxepine. No activity with geranylgeranyl diphosphate. The enzyme forms (+)-corvol ether B and (+)-corvol ether A in the ratio of 3:1 Kitasatospora setae DSM 43861 (+)-corvol ether B + diphosphate
-
 ?
additional information incubation of the purified enzyme with geranyl diposphate yields small amounts of several acyclic and cyclic monoterpenes, with linalool as the main product Kitasatospora setae ?
-
 ?
additional information incubation of the purified enzyme with geranyl diposphate yields small amounts of several acyclic and cyclic monoterpenes, with linalool as the main product Kitasatospora setae DSM 43861 ?
-
 ?

Synonyms

Synonyms Comment Organism
KSE_12950
-
 Kitasatospora setae