Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sodium phosphate | stimulating at low concentrations | Mentha sp. |
General Stability | Organism |
---|---|
unstable in crude secretory cell extract, stable after DEAE-cellulose chromatography | Mentha sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyl bromide | - |
Mentha sp. | |
diethyl dicarbonate | - |
Mentha sp. | |
linalyl sulfonium ion | - |
Mentha sp. | |
N-ethylmaleimide | reversible by cysteine treatment | Mentha sp. | |
p-hydroxymercuribenzoate | pretreatment with geranyl diphosphate and MnCl2 prevents inhibition | Mentha sp. | |
Sodium diphosphate | Ki: 1 mM | Mentha sp. | |
Sodium phosphate | Ki: 30 mM | Mentha sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
neryl diphosphate | - |
Mentha sp. | |
0.0018 | - |
geranyl diphosphate | - |
Mentha sp. | |
0.0019 | - |
(3R)-linalyl diphosphate | - |
Mentha sp. | |
0.0023 | - |
(3S)-linalyl diphosphate | - |
Mentha sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mn2+ preferred | Mentha sp. | |
Mn2+ | required, optimum activity with 2 mM, inhibitory at higher concentrations | Mentha sp. | |
additional information | Ca2+, Cd2+, Co2+, Cu2+, Fe2+, Ni2+, Zn2+ activated less than 5% | Mentha sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | Mentha sp. | - |
(-)-(4S)-limonene + diphosphate | small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha sp. | - |
M. x piperita | - |
Purification (Comment) | Organism |
---|---|
partial | Mentha sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Mentha sp. | - |
Storage Stability | Organism |
---|---|
4°C, stable for 1 week without loss of activity after DEAE-cellulose chromatography | Mentha sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-linalyl diphosphate | - |
Mentha sp. | ? | - |
? | |
(3S)-linalyl diphosphate | - |
Mentha sp. | ? | - |
? | |
geranyl diphosphate | - |
Mentha sp. | (-)-(4S)-limonene + diphosphate | small amounts of (-)-alpha-pinene, (-)-beta-pinene and myrcene as side products | ? | |
neryl diphosphate | - |
Mentha sp. | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Mentha sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.1 | 7.2 | half maximum activity at both pH | Mentha sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
Sodium diphosphate | - |
Mentha sp. | |
30 | - |
Sodium phosphate | - |
Mentha sp. |