Cloned (Comment) | Organism |
---|---|
overexpressed heterologously in Streptomyces lividans 66 | Actinoplanes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | assay bufer contains Co2+ | Actinoplanes sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sedoheptulose 7-phosphate | Actinoplanes sp. | the cyclization product, 2-epi-5-epi-valiolone is a precursor of the valienamine moiety of acarbose | 2-epi-5-epi-valiolone + phosphate | - |
? | |
D-sedoheptulose 7-phosphate | Actinoplanes sp. 50/110 | the cyclization product, 2-epi-5-epi-valiolone is a precursor of the valienamine moiety of acarbose | 2-epi-5-epi-valiolone + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinoplanes sp. | Q9ZAE9 | - |
- |
Actinoplanes sp. 50/110 | Q9ZAE9 | - |
- |
Storage Stability | Organism |
---|---|
-20°C, AcbC extract can be stored for 3 months without major loss of activity | Actinoplanes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sedoheptulose 7-phosphate | the cyclization product, 2-epi-5-epi-valiolone is a precursor of the valienamine moiety of acarbose | Actinoplanes sp. | 2-epi-5-epi-valiolone + phosphate | - |
? | |
D-sedoheptulose 7-phosphate | no activity with ido-heptulose 7-phosphate | Actinoplanes sp. | 2-epi-5-epi-valiolone + phosphate | - |
? | |
D-sedoheptulose 7-phosphate | the cyclization product, 2-epi-5-epi-valiolone is a precursor of the valienamine moiety of acarbose | Actinoplanes sp. 50/110 | 2-epi-5-epi-valiolone + phosphate | - |
? | |
D-sedoheptulose 7-phosphate | no activity with ido-heptulose 7-phosphate | Actinoplanes sp. 50/110 | 2-epi-5-epi-valiolone + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AbcC | - |
Actinoplanes sp. |
C7-cyclitol synthase | - |
Actinoplanes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Actinoplanes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Actinoplanes sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | assay buffer contains NAD+ | Actinoplanes sp. |