Cloned (Comment) | Organism |
---|---|
gene cotB2, recombinant expression of His-tagged mutant enzymes in Escherichia coli strain BL21(DE3) | Streptomyces melanosporofaciens |
Protein Variants | Comment | Organism |
---|---|---|
F107A | site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different product cembrane A instead of cyclooctat-9-en-7-ol | Streptomyces melanosporofaciens |
additional information | mutational analysis of the atypical aspartate-rich motif of CotB2 (EC 4.2.3.146). The substrate specificity of the CotB2 mutants can be completely changed. Proposed cyclization mechanism for CotB2 and its mutants, overview | Streptomyces melanosporofaciens |
W186L | site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different products cembrane A and 3,7,18-dolabellatriene and only low amounts of cyclooctat-9-en-7-ol | Streptomyces melanosporofaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptomyces melanosporofaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Streptomyces melanosporofaciens | - |
cembrene A + diphosphate | - |
? | |
geranylgeranyl diphosphate | Streptomyces melanosporofaciens MI614-43F2 | - |
cembrene A + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces melanosporofaciens | - |
- |
- |
Streptomyces melanosporofaciens MI614-43F2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptomyces melanosporofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Streptomyces melanosporofaciens | cembrene A + diphosphate | - |
? | |
geranylgeranyl diphosphate | activity of enzyme mutants F107A and W186L, not of wild-type enzyme | Streptomyces melanosporofaciens | cembrene A + diphosphate | - |
? | |
geranylgeranyl diphosphate | - |
Streptomyces melanosporofaciens MI614-43F2 | cembrene A + diphosphate | - |
? | |
geranylgeranyl diphosphate | activity of enzyme mutants F107A and W186L, not of wild-type enzyme | Streptomyces melanosporofaciens MI614-43F2 | cembrene A + diphosphate | - |
? | |
additional information | the mutants F107A and W186L follow a different reaction mechanism compared to wild-type CotB2, and produce the different product cembrane A | Streptomyces melanosporofaciens | ? | - |
? | |
additional information | the mutants F107A and W186L follow a different reaction mechanism compared to wild-type CotB2, and produce the different product cembrane A | Streptomyces melanosporofaciens MI614-43F2 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces melanosporofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces melanosporofaciens |