Literature summary for 4.2.3.147 extracted from

Morrone, D.; Xu, M.; Fulton, D.B.; Determan, M.K.; Peters, R.J.
Increasing complexity of a diterpene synthase reaction with a single residue switch (2008), J. Am. Chem. Soc., 130, 5400-5401.

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Protein Variants

Protein Variants	Comment	Organism
T696I	mutation leads to a dramatic increase in reaction complexity. Product outcome is shifted from the tricyclic pimara-7,15-diene to a rearranged tetracycle, aphidicol-15-ene. Products are about 20% pimara-7,15-diene plus 80% aphidicol-15-ene	Oryza sativa

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-copalyl diphosphate	-	Oryza sativa	aphidicol-15-ene + phosphate	products of mutant T696I are about 20% pimara-7,15-diene plus 80% aphidicol-15-ene, no product of wild-type	?
(+)-copalyl diphosphate	-	Oryza sativa	pimara-7,15-diene + diphosphate	product of wild-type	?

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Release 2023.1 (January 2023)