BRENDA - Enzyme Database
show all sequences of 4.2.3.142

Haring, M.A. and Schuurink, R.C. Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative

Bleeker, P.M.; Mirabella, R.; Diergaarde, P.J.; Vandoorn, A.; Tissier, A.; Kant, M.R.; Prins, M.; de Vos, M.; Proc. Natl. Acad. Sci. USA 109, 20124-20129 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
construction of an interspecific cross between Solanum lycopersicum cv Moneymaker and the wild tomato Solanum habrochaites PI127826 leads to production of 7-epizingiberene in a range of concentrations up to PI127826 levels in the F2 plants. Plants also accumulate monoterpenes and/or other sesquiterpenes at high concentrations. Data show a correlation between the concentration of 7-epizingiberene on the leaf surface and the defense against whiteflies. Stable expression of short chain cis-prenyltransferase zFPS and 7-epizingiberene synthase in Solanum lycopersicum results in accumulation of 7-epizingiberene. The production of 7-epizingiberene has no repellent effect on spider mites in a choice assay, but has a strong effect on their fecundity. Mites placed on leaf disks of transgenic plants exhibit a 40% higher mortality and, after 4 d, an 81% reduction in the number of eggs
Solanum habrochaites
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
(2Z,6Z)-farnesyl diphosphate
pH not specified in the publication, temperature not specified in the publication
Solanum habrochaites
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Solanum habrochaites
9536
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
84000
-
x * 84000, calculated
Solanum habrochaites
Organism
Organism
UniProt
Commentary
Textmining
Solanum habrochaites
-
-
-
Solanum habrochaites PI127826
-
-
-
Solanum lycopersicum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
trichome
-
Solanum habrochaites
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2Z,6Z)-farnesyl diphosphate
exclusively uses (2Z,6Z)-farnesyl-diphosphate
720937
Solanum habrochaites
7-epizingiberene + diphosphate
plus trace amounts of beta-sesquiphellandrene and curcumene
-
-
?
(2Z,6Z)-farnesyl diphosphate
exclusively uses (2Z,6Z)-farnesyl-diphosphate
720937
Solanum habrochaites PI127826
7-epizingiberene + diphosphate
plus trace amounts of beta-sesquiphellandrene and curcumene
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 84000, calculated
Solanum habrochaites
Synonyms
Synonyms
Commentary
Organism
ShZIS
-
Solanum lycopersicum
Zis
-
Solanum habrochaites
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.29
-
(2Z,6Z)-farnesyl diphosphate
pH not specified in the publication, temperature not specified in the publication
Solanum habrochaites
Application (protein specific)
Application
Commentary
Organism
agriculture
construction of an interspecific cross between Solanum lycopersicum cv Moneymaker and the wild tomato Solanum habrochaites PI127826 leads to production of 7-epizingiberene in a range of concentrations up to PI127826 levels in the F2 plants. Plants also accumulate monoterpenes and/or other sesquiterpenes at high concentrations. Data show a correlation between the concentration of 7-epizingiberene on the leaf surface and the defense against whiteflies. Stable expression of short chain cis-prenyltransferase zFPS and 7-epizingiberene synthase in Solanum lycopersicum results in accumulation of 7-epizingiberene. The production of 7-epizingiberene has no repellent effect on spider mites in a choice assay, but has a strong effect on their fecundity. Mites placed on leaf disks of transgenic plants exhibit a 40% higher mortality and, after 4 d, an 81% reduction in the number of eggs
Solanum habrochaites
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
(2Z,6Z)-farnesyl diphosphate
pH not specified in the publication, temperature not specified in the publication
Solanum habrochaites
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Solanum habrochaites
9536
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
84000
-
x * 84000, calculated
Solanum habrochaites
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
trichome
-
Solanum habrochaites
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2Z,6Z)-farnesyl diphosphate
exclusively uses (2Z,6Z)-farnesyl-diphosphate
720937
Solanum habrochaites
7-epizingiberene + diphosphate
plus trace amounts of beta-sesquiphellandrene and curcumene
-
-
?
(2Z,6Z)-farnesyl diphosphate
exclusively uses (2Z,6Z)-farnesyl-diphosphate
720937
Solanum habrochaites PI127826
7-epizingiberene + diphosphate
plus trace amounts of beta-sesquiphellandrene and curcumene
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 84000, calculated
Solanum habrochaites
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.29
-
(2Z,6Z)-farnesyl diphosphate
pH not specified in the publication, temperature not specified in the publication
Solanum habrochaites
General Information
General Information
Commentary
Organism
physiological function
stable expression of short chain cis-prenyltransferase zFPS and 7-epizingiberene synthase in Solanum lycopersicum results in accumulation of 7-epizingiberene. The production of 7-epizingiberene has no repellent effect on spider mites in a choice assay, but has a strong effect on their fecundity. Mites placed on leaf disks of transgenic plants exhibit a 40% higher mortality and, after 4 d, an 81% reduction in the number of eggs
Solanum habrochaites
General Information (protein specific)
General Information
Commentary
Organism
physiological function
stable expression of short chain cis-prenyltransferase zFPS and 7-epizingiberene synthase in Solanum lycopersicum results in accumulation of 7-epizingiberene. The production of 7-epizingiberene has no repellent effect on spider mites in a choice assay, but has a strong effect on their fecundity. Mites placed on leaf disks of transgenic plants exhibit a 40% higher mortality and, after 4 d, an 81% reduction in the number of eggs
Solanum habrochaites
Other publictions for EC 4.2.3.142
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720937
Bleeker
Haring, M.A. and Schuurink, R. ...
Solanum habrochaites, Solanum habrochaites PI127826, Solanum lycopersicum
Proc. Natl. Acad. Sci. USA
109
20124-20129
2012
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5
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2
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1
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1
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730612
Gonzales-Vigil
Evolution of TPS20-related ter ...
Solanum habrochaites
Plant J.
71
921-935
2012
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