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Literature summary for 4.2.3.140 extracted from

  • Zerbe, P.; Chiang, A.; Yuen, M.; Hamberger, B.; Hamberger, B.; Draper, J.A.; Britton, R.; Bohlmann, J.
    Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production (2012), J. Biol. Chem., 287, 12121-12131.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.140

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed in Escherichia coli Abies balsamea

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling based on PDB entry 3p5pA. The active site residues Asp-48, Leu617, Phe696, and Gly723 are potentially important for the specificity Abies balsamea

Protein Variants

Protein Variants Comment Organism
D402A/D404A mutation results in complete loss of activity Abies balsamea
D402A/D404A monofunctional class II protein variant, complete loss of enzymatic activity with geranylgeranyl diphosphate as substrate Abies balsamea
D621A monofunctional class I protein variant, mutant produces trace amounts of copalyl diphosphate plus labda-13-en-8,15-diol Abies balsamea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Abies balsamea 9507
-

Organism

Organism UniProt Comment Textmining
Abies balsamea H8ZM73
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Abies balsamea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate formation of cis-abienol proceeds via cyclization and hydroxylation at carbon C-8 of a postulated carbocation intermediate in the class II active site, followed by cleavage of the diphosphate group and termination of the reaction sequence without further cyclization in the class I active site Abies balsamea cis-abienol + diphosphate
-
 ?
additional information using (E,E,E)-geranylgeranyl diphosphate as a substrate AbdiTPS4 shows a unique single-peak product profile, which corresponds to cis-abienol 4 Abies balsamea ?
-
 ?
additional information bifunctional class I/II enzyme Abies balsamea ?
-
 ?

Synonyms

Synonyms Comment Organism
AbCAS
-
 Abies balsamea
AbdiTPS4
-
 Abies balsamea
CAS
-
 Abies balsamea
cis-abienol synthase
-
 Abies balsamea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Abies balsamea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Abies balsamea