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Literature summary for 4.2.3.138 extracted from
- Molecular cloning and characterization of (+)-epi-alpha-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis (2012), Arch. Biochem. Biophys., 527, 37-44.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in yeast and Escherichia coli. The engineered yeast can synthesize a significant amount (0.3 mg per ml) of alpha-bisabolol in shake-flask cultivation | Phyla dulcis |
| expression in Saccharomyces cerevisiae and Escherichia coli | Phyla dulcis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C | Phyla dulcis |  |
| 0.0048 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 30°C | Phyla dulcis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phyla dulcis | - |
- |
- |
| Phyla dulcis | J7LH11 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Phyla dulcis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Phyla dulcis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | - |
Phyla dulcis | (+)-epi-alpha-bisabolol + diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + H2O | - |
Phyla dulcis | (+)-epi-alpha-bisabolol + diphosphate | single terpene product in vitro | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (+)-epi-alpha-bisabolol synthase | - |
Phyla dulcis |
| LdTPS8 | - |
Phyla dulcis |
| TPS8 | - |
Phyla dulcis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phyla dulcis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C | Phyla dulcis | |
| 0.04 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 30°C | Phyla dulcis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Phyla dulcis |
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