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Literature summary for 4.2.3.135 extracted from
- Combinatorial engineering of hybrid mevalonate pathways in Escherichia coli for protoilludene production (2016), Microb. Cell Fact., 15, 14 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | protoilludene is a valuable sesquiterpene and serves as a precursor for several medicinal compounds and antimicrobial chemicals. It can be synthesized by heterologous overexpression of protoilludene synthase in Escherichia coli with coexpression of mevalonate or methylerythritol-phosphate pathway enzymes, and farnesyl diphosphate synthase as a cell factory for protoilludene production | Omphalotus olearius |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene OMP7, functional overexpression of codon-optimized gene in Escherichia coli strain DH5alpha, coexpression with Escherichia coli farnesyl diphosphate synthase IspA | Omphalotus olearius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | protoilludene is successfully produced in Escherichia coli by overexpression of a hybrid exogenous mevalonate pathway, endogenous farnesyl diphosphate synthase (IspA), and protoilludene synthase (OMP7) of Omphalotus olearius | Omphalotus olearius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | Omphalotus olearius | - |
DELTA6-protoilludene + diphosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Omphalotus olearius | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Omphalotus olearius | DELTA6-protoilludene + diphosphate | - |
? | |
| additional information | product formation is identified and quantified by GC-MS analysis | Omphalotus olearius | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OMP7 | - |
Omphalotus olearius |
| protoilludene synthase | - |
Omphalotus olearius |
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Release 2023.1 (January 2023)
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