Literature summary for 4.2.3.132 extracted from

Peters, R.J.; Flory, J.E.; Jetter, R.; Ravn, M.M.; Lee, H.J.; Coates, R.M.; Croteau, R.B.
Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme (2000), Biochemistry, 39, 15592-15602.

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Cloned(Commentary)

Cloned (Comment)	Organism
truncated abietadiene synthase is expressed in Escherichia coli BL21(DE3) cells	Abies grandis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00035	-	(+)-copalyl diphosphate	in 50 mM HEPES, pH 7.2, 100 mM KCl, 7.5 mM MgCl2, 0.02 mM MnCl2, at 30°C	Abies grandis

Organism

Organism	UniProt	Comment	Textmining
Abies grandis	-	grand fir	-

Purification (Commentary)

Purification (Comment)	Organism
type II ceramic hydroxyapatite column chromatography and POROS HQ/M column chromatography	Abies grandis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-copalyl diphosphate	the enzyme converts geranylgeranyl diphosphate and the intermediate (+)-copalyl diphosphate to a nearly equal mixture of abietadiene (31%), levopimaradiene (34%), and neoabietadiene (28%), as well as to three minor products pimara-8(14),15-diene (3%), palustradiene (2%), and sandaracopimaradiene (2%)	Abies grandis	?	-	?

Synonyms

Synonyms	Comment	Organism
abietadiene synthase	cf. EC 4.2.3.18	Abies grandis
NAS	-	Abies grandis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.75	-	(+)-copalyl diphosphate	in 50 mM HEPES, pH 7.2, 100 mM KCl, 7.5 mM MgCl2, 0.02 mM MnCl2, at 30°C	Abies grandis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2000	-	(+)-copalyl diphosphate	in 50 mM HEPES, pH 7.2, 100 mM KCl, 7.5 mM MgCl2, 0.02 mM MnCl2, at 30°C	Abies grandis

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Release 2023.1 (January 2023)