Cloned (Comment) | Organism |
---|---|
gene KSL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time PCR enzyme expression analysis, recombinant expression of HA-His-tandem-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL, functional transient recombinant coexpression of Salvia fruticosa (+)-copalyl diphosphate sythase (SfCPS) and Salvia fruticosa ent-kaurene synthase-like (SfKSL) in Nicotiana benthamiana via transfection by Agrobacterium tumefaciens leading to production of miltiradiene in planta, NMR product analysis | Salvia fruticosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Salvia fruticosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl diphosphate | Salvia fruticosa | - |
miltiradiene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia fruticosa | A0A0C5QH30 | genotypes France, Vrysses, and Kavoussi | - |
Purification (Comment) | Organism |
---|---|
recombinant HA-His-tandem-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography | Salvia fruticosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young leaves show higher accumulation of SfKSL transcripts, when compared to the fully expanded leaves, consistent with the trichome and developmental regulation of carnosic acid build-up | Salvia fruticosa | - |
additional information | real-time PCR analysis demonstrates higher expression level of SfKSL in isolated trichomes than in leaves without trichomes | Salvia fruticosa | - |
trichome | glandular, transcriptome analysis, overview | Salvia fruticosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl diphosphate | - |
Salvia fruticosa | miltiradiene + diphosphate | - |
? | |
additional information | synthesis of miltiradiene through the enzyme is confirmed by 1D and 2D NMR analyses, GC-MS analysis, overview | Salvia fruticosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ent-kaurene synthase-like | - |
Salvia fruticosa |
KSL | - |
Salvia fruticosa |
SfKSL | - |
Salvia fruticosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salvia fruticosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Salvia fruticosa |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme SfKSL is responsible for the formation of the carnosic acid precursor miltiradiene | Salvia fruticosa |