Literature summary for 4.2.3.131 extracted from

Su, P.; Guan, H.; Zhao, Y.; Tong, Y.; Xu, M.; Zhang, Y.; Hu, T.; Yang, J.; Cheng, Q.; Gao, L.; Liu, Y.; Zhou, J.; Peters, R.J.; Huang, L.; Gao, W.
Identification and functional characterization of diterpene synthases for triptolide biosynthesis from Tripterygium wilfordii (2018), Plant J., 93, 50-65 .

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Cloned(Commentary)

Cloned (Comment)	Organism
TwTPS27v2, phylogenetic analysis and tree, coexpression of TwTPS27v2 with either copalyl diphosphate synthases TwTPS7v2 or TwTPS9v2 in appropriately engineered Saccharomyces cerevisiae strain, or recombinant coexpression in Escherichia coli, the combination with TwTPS7v2 produces miltiradiene, recombinant expression oof GFP-tagged enzyme TwTPS27v2 in Nicotiana benthamiana chloroplasts, quantitative RT-PCR expression analysis	Tripterygium wilfordii

Cloned (Comment)

Organism

TwTPS27v2, phylogenetic analysis and tree, coexpression of TwTPS27v2 with either copalyl diphosphate synthases TwTPS7v2 or TwTPS9v2 in appropriately engineered Saccharomyces cerevisiae strain, or recombinant coexpression in Escherichia coli, the combination with TwTPS7v2 produces miltiradiene, recombinant expression oof GFP-tagged enzyme TwTPS27v2 in Nicotiana benthamiana chloroplasts, quantitative RT-PCR expression analysis

Tripterygium wilfordii

Protein Variants

Protein Variants	Comment	Organism
additional information	RNAi targeting of TwTPS27v2 is carried out reduces the transcript level of TwTPS27v2 to about 30%, with no reduction in the levels of the related TPS-b subfamily members TwTPS22v2, TwTPS23v2, TwTPS32 and TwTPS33, triptolide accumulation is decreased by about 40%	Tripterygium wilfordii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Tripterygium wilfordii	9507	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(+)-copalyl diphosphate	Tripterygium wilfordii	-	miltiradiene + diphosphate	-	?
additional information	Tripterygium wilfordii	miltiradiene spontaneously forms abietatriene	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Tripterygium wilfordii	A0A1C7AAM8	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	-	Tripterygium wilfordii	-
leaf	transcriptome analysis	Tripterygium wilfordii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-copalyl diphosphate	-	Tripterygium wilfordii	miltiradiene + diphosphate	-	?
additional information	miltiradiene spontaneously forms abietatriene	Tripterygium wilfordii	?	-	?

Synonyms

Synonyms	Comment	Organism
TwTPS27v2	-	Tripterygium wilfordii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Tripterygium wilfordii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Tripterygium wilfordii

General Information

General Information	Comment	Organism
evolution	the enzyme TwTPS27v2 belongs to the terpene synthases superfamily and the TPS-b subfamily	Tripterygium wilfordii
malfunction	RNA interference targeting either both of the copalyl diphosphate synthases, TwTPS7v2 and TwTPS9v2, or the subsequently acting miltiradiene synthase, TwTPS27v2, leads to decreased production of triptolide	Tripterygium wilfordii
metabolism	miltiradiene is the relevant precursor in the biosynthesis of tripolide. Triptolide production is induced by methyl jasmonate. ENzyme TwTPS7v2, TwTPS9v2 and TwTPS27v2 are involved in triptolide biosynthesis	Tripterygium wilfordii
physiological function	miltiradiene is the relevant precursor in the biosynthesis of tripolide. Triptolide and celastrol are the main active diterpenoid and triterpenoid ingredients, respectively, that exhibit impressive and effective anti-inflammatory, immunosuppressive and anti-tumor activities and make Tripterygium wilfordii a medicinal plant	Tripterygium wilfordii