Cloned (Comment) | Organism |
---|---|
cloning of full-length cDNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant transient Agrobacterium tumefaciens-mediated coexpression of Isodon rubescens gene TPS4, encoding miltiradiene synthase, with Isodon rubescens gene TPS3, encoding (+)-copalyl diphosphate synthase in Nicotiana benthamiana results in production of miltiradiene, while coexpression with Isodon rubescens gene TPS2, encoding ent-copalyl diphosphate synthase, does not lead to product formation | Isodon rubescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Isodon rubescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl diphosphate | Isodon rubescens | - |
miltiradiene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Isodon rubescens | A0A1W6QDI7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | high expression levels of TPS4 in leaves | Isodon rubescens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl diphosphate | - |
Isodon rubescens | miltiradiene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IrTPS4 | - |
Isodon rubescens |
TPS4 | - |
Isodon rubescens |