Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.13 extracted from

  • Jin, Z.; Kwon, M.; Lee, A.R.; Ro, D.K.; Wungsintaweekul, J.; Kim, S.U.
    Molecular cloning and functional characterization of three terpene synthases from unripe fruit of black pepper (Piper nigrum) (2018), Arch. Biochem. Biophys., 638, 35-40 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene PnTPS2, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain EPY300. PnTPS2-transformed yeast cells accumulate delta-cadinol as a major product (91%) and smaller amounts of alpha-cadinene (2%) and delta-cadinene (7%) Piper nigrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0184
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Piper nigrum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Piper nigrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Piper nigrum
-
(+)-delta-cadinene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Piper nigrum A0A1V0E4A6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Piper nigrum
-
leaf
-
Piper nigrum
-
root
-
Piper nigrum
-
stem
-
Piper nigrum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Piper nigrum (+)-delta-cadinene + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 64600, about, sequence calculation Piper nigrum

Synonyms

Synonyms Comment Organism
cadinene synthase UniProt Piper nigrum
cadinol/cadinene synthase
-
Piper nigrum
PnCO/CDS
-
Piper nigrum
PnTPS2
-
Piper nigrum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.694
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Piper nigrum

General Information

General Information Comment Organism
metabolism possible mechanism of sesquiterpene synthases from Piper nigrum. The reaction pathway is based on 1,10-ring closure pathway including enzyme PnCO/CDS Piper nigrum
additional information PnTPS2-transformed yeast cells accumulate delta-cadinol as a major product (91%) and smaller amounts of alpha-cadinene (2%) and delta-cadinene (7%). Recombinant expression of Piper nigrum germacrene D synthase (PnGDS) in yeast yields increased beta-cadinene and alpha-copaene contents Piper nigrum
physiological function the enzyme is involved in biosynthesis of beta-copaene and beta-cadinene (EC 4.2.3.13). In the unripe fruit, thirteen sesquiterpenes including beta-caryophyllene, delta-elemene, alpha-copaene, cubebene, alpha-humulene, delta-cadinol, and gamma- and delta-cadinenes are found. beta-Caryophyllene is the most abundant compound among the observed sesquiterpenes (65.8% of the total), followed by copaene (8.3%). During fruit ripening, the contents of the sesquiterpenes derived from germacrene D, such as cubebene, copaene, cubeol, and germacrene D-4?-ol, are progressively declined, most likely due to diminished total germacrene D synthase activity Piper nigrum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
37.7
-
(2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Piper nigrum