Cloned (Comment) | Organism |
---|---|
gene PnTPS2, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain EPY300. PnTPS2-transformed yeast cells accumulate delta-cadinol as a major product (91%) and smaller amounts of alpha-cadinene (2%) and delta-cadinene (7%) | Piper nigrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0184 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Piper nigrum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Piper nigrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Piper nigrum | - |
(+)-delta-cadinene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Piper nigrum | A0A1V0E4A6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Piper nigrum | - |
leaf | - |
Piper nigrum | - |
root | - |
Piper nigrum | - |
stem | - |
Piper nigrum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Piper nigrum | (+)-delta-cadinene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64600, about, sequence calculation | Piper nigrum |
Synonyms | Comment | Organism |
---|---|---|
cadinene synthase | UniProt | Piper nigrum |
cadinol/cadinene synthase | - |
Piper nigrum |
PnCO/CDS | - |
Piper nigrum |
PnTPS2 | - |
Piper nigrum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.694 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Piper nigrum |
General Information | Comment | Organism |
---|---|---|
metabolism | possible mechanism of sesquiterpene synthases from Piper nigrum. The reaction pathway is based on 1,10-ring closure pathway including enzyme PnCO/CDS | Piper nigrum |
additional information | PnTPS2-transformed yeast cells accumulate delta-cadinol as a major product (91%) and smaller amounts of alpha-cadinene (2%) and delta-cadinene (7%). Recombinant expression of Piper nigrum germacrene D synthase (PnGDS) in yeast yields increased beta-cadinene and alpha-copaene contents | Piper nigrum |
physiological function | the enzyme is involved in biosynthesis of beta-copaene and beta-cadinene (EC 4.2.3.13). In the unripe fruit, thirteen sesquiterpenes including beta-caryophyllene, delta-elemene, alpha-copaene, cubebene, alpha-humulene, delta-cadinol, and gamma- and delta-cadinenes are found. beta-Caryophyllene is the most abundant compound among the observed sesquiterpenes (65.8% of the total), followed by copaene (8.3%). During fruit ripening, the contents of the sesquiterpenes derived from germacrene D, such as cubebene, copaene, cubeol, and germacrene D-4?-ol, are progressively declined, most likely due to diminished total germacrene D synthase activity | Piper nigrum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.7 | - |
(2E,6E)-farnesyl diphosphate | pH and temperature not specified in the publication | Piper nigrum |