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Literature summary for 4.2.3.13 extracted from
- Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes (2009), BMC Plant Biol., 9, 86.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain EPY300 as a thioredoxin-fusion protein | Helianthus annuus |
| thioredoxin-fusion protein expressed in Escherichia coli, expressed in Saccharomyces cerevisiae EPY300 | Helianthus annuus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64200 | - |
x * 64200, calculated | Helianthus annuus |
| 64200 | - |
x * 64200, calculated from amino acid sequence | Helianthus annuus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | Q4U3F6 | - |
- |
| Helianthus annuus | Q4U3F6 | isoform TPS2; cultivar HA300 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA affinity chromatography | Helianthus annuus |
| Ni-NTA affinity column chromatography | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| glandular trichome | - |
Helianthus annuus | - |
| leaf | only young leaf | Helianthus annuus | - |
| trichome | - |
Helianthus annuus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Helianthus annuus | delta-cadinene + diphosphate | delta-cadinene is one of the two main products, while alpha-muuorlene, beta-caryophyllene, alpha-humulene and alpha-copaene are minor products | ? |  |
| additional information | - |
Helianthus annuus | ? | products (in vivo expression in Saccharomyces cerevisiae): delta-cadinene and a compound with a cadinene skeleton, minor products: alpha-muurolene, beta-caryophyllene, alpha-humulene and alpha-copaene | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64200, calculated | Helianthus annuus |
| ? | x * 64200, calculated from amino acid sequence | Helianthus annuus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cadinene synthase | - |
Helianthus annuus |
| sesquiterpene synthase | - |
Helianthus annuus |
| TPS2 | isoform | Helianthus annuus |
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