Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Gossypium arboreum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Gossypium arboreum |
Protein Variants | Comment | Organism |
---|---|---|
D307A | aspartate-rich motif interacts with putative Mg2+ | Gossypium arboreum |
D308A | aspartate-rich motif interacts with putative Mg2+ | Gossypium arboreum |
D311A | aspartate-rich motif interacts with putative Mg2+ | Gossypium arboreum |
D451A | aspartate-rich motif interacts with putative Mg2+ | Gossypium arboreum |
D452A | aspartate-rich motif interacts with putative Mg2+ | Gossypium arboreum |
E455A | aspartate-rich motif interacts with putative Mg2+ | Gossypium arboreum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-fluorofarnesyl diphosphate | - |
Gossypium arboreum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
(2E,6Z)-farnesyl diphosphate | D451A mutant protein | Gossypium arboreum | |
0.0031 | - |
(2E,6Z)-farnesyl diphosphate | D452A mutant protein | Gossypium arboreum | |
0.0032 | - |
(2E,6Z)-farnesyl diphosphate | wild-type protein | Gossypium arboreum | |
0.043 | - |
(2E,6Z)-farnesyl diphosphate | D308A mutant protein | Gossypium arboreum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Gossypium arboreum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
1 * 64000, gel filtration, SDS-PAGE | Gossypium arboreum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium arboreum | Q39761 | - |
- |
Purification (Comment) | Organism |
---|---|
soluble at pH 11.6, ion exchange chromatography (DEAE), gel filtration | Gossypium arboreum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6Z)-farnesyl diphosphate | - |
Gossypium arboreum | (+)-delta-cadinene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, gel filtration, SDS-PAGE | Gossypium arboreum |
Synonyms | Comment | Organism |
---|---|---|
(+)-delta-cadinene synthase isoenzyme XC1 | - |
Gossypium arboreum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
(2E,6Z)-farnesyl diphosphate | wild-type protein | Gossypium arboreum | |
0.012 | - |
(2E,6Z)-farnesyl diphosphate | D308A mutant protein | Gossypium arboreum | |
0.014 | - |
(2E,6Z)-farnesyl diphosphate | D452A mutant protein | Gossypium arboreum | |
0.043 | - |
(2E,6Z)-farnesyl diphosphate | D451A mutant protein | Gossypium arboreum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.03 | - |
0.01 mM farnesyl diphosphate | Gossypium arboreum | 2-fluorofarnesyl diphosphate |