Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.13 extracted from

  • ennadios, H.A.; Gonzalez, V.; Di Costanzo, L.; Li, A.; Yu, F.; Miller, D.J.; Allemann, R.K.; Christianson, D.W.
    Crystal structure of (+)-delta-cadinene synthase from Gossypium arboreum and evolutionary divergence of metal binding motifs for catalysis (2009), Biochemistry, 48, 6175-6183.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Gossypium arboreum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Gossypium arboreum

Protein Variants

Protein Variants Comment Organism
D307A aspartate-rich motif interacts with putative Mg2+ Gossypium arboreum
D308A aspartate-rich motif interacts with putative Mg2+ Gossypium arboreum
D311A aspartate-rich motif interacts with putative Mg2+ Gossypium arboreum
D451A aspartate-rich motif interacts with putative Mg2+ Gossypium arboreum
D452A aspartate-rich motif interacts with putative Mg2+ Gossypium arboreum
E455A aspartate-rich motif interacts with putative Mg2+ Gossypium arboreum

Inhibitors

Inhibitors Comment Organism Structure
2-fluorofarnesyl diphosphate
-
Gossypium arboreum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
(2E,6Z)-farnesyl diphosphate D451A mutant protein Gossypium arboreum
0.0031
-
(2E,6Z)-farnesyl diphosphate D452A mutant protein Gossypium arboreum
0.0032
-
(2E,6Z)-farnesyl diphosphate wild-type protein Gossypium arboreum
0.043
-
(2E,6Z)-farnesyl diphosphate D308A mutant protein Gossypium arboreum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Gossypium arboreum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
1 * 64000, gel filtration, SDS-PAGE Gossypium arboreum

Organism

Organism UniProt Comment Textmining
Gossypium arboreum Q39761
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble at pH 11.6, ion exchange chromatography (DEAE), gel filtration Gossypium arboreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6Z)-farnesyl diphosphate
-
Gossypium arboreum (+)-delta-cadinene + diphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64000, gel filtration, SDS-PAGE Gossypium arboreum

Synonyms

Synonyms Comment Organism
(+)-delta-cadinene synthase isoenzyme XC1
-
Gossypium arboreum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
(2E,6Z)-farnesyl diphosphate wild-type protein Gossypium arboreum
0.012
-
(2E,6Z)-farnesyl diphosphate D308A mutant protein Gossypium arboreum
0.014
-
(2E,6Z)-farnesyl diphosphate D452A mutant protein Gossypium arboreum
0.043
-
(2E,6Z)-farnesyl diphosphate D451A mutant protein Gossypium arboreum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.03
-
0.01 mM farnesyl diphosphate Gossypium arboreum 2-fluorofarnesyl diphosphate