Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of the CAD1-A isoenzyme, 80% of homology with CAD1-C isoenzyme | Gossypium arboreum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
2-trans,6-trans-farnesyl diphosphate | isoenzyme CAD1-A, pH 8.7, 30°C | Gossypium arboreum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2.5 mM MgCl2 required for optimal activity, enzyme form CAD1-A | Gossypium arboreum | |
Mg2+ | 15 mM MgCl2 required for optimal activity, enzyme form CAD1-C | Gossypium arboreum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64060 | - |
calculated from DNA sequence, CAD1-A isozyme | Gossypium arboreum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium arboreum | - |
cv. Nanking | - |
Gossypium arboreum | - |
two enzyme forms CAD1-A and CAD1-C1 | - |
Gossypium arboreum | - |
tree cotton | - |
Purification (Comment) | Organism |
---|---|
CAD1-A isoenzyme | Gossypium arboreum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
CAD1-A isozyme, expressed in Escherichia coli | Gossypium arboreum |
0.036 | - |
CAD1-A isozyme, removal of 15 amino acids by thrombin cleavage | Gossypium arboreum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | - |
Gossypium arboreum | (+)-delta-cadinene + diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | enzyme form CAD1-C | Gossypium arboreum |
8.7 | - |
enzyme form CAD1-A | Gossypium arboreum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.7 | 62% of maximal activity at pH 6.8, enzyme form CAD1-A | Gossypium arboreum |
7 | 8.7 | 60% of maximal activity at pH 8.7, enzyme form CAD1-C | Gossypium arboreum |