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Literature summary for 4.2.3.128 extracted from

  • Lee, S.; Chappell, J.
    Biochemical and genomic characterization of terpene synthases in Magnolia grandiflora (2008), Plant Physiol., 147, 1017-1033.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Mg25, DNA and amino acid sequence determination and analysis, Mg25 has one single intron located near the 5' terminus of the gene, expression analysis and phylogenetic analysis Magnolia grandiflora

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, maximal activities at Mg2+ concentrations of 1 to 10 mM Magnolia grandiflora
Mn2+ activates, naximally at concentrations of 1 mM or less Magnolia grandiflora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Magnolia grandiflora Mg25 converts farnesyl diphosphate predominantly to beta-cubebene beta-cubebene + diphosphate
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?
additional information Magnolia grandiflora the dominant reaction products generated by Mg25 are beta-cubebene with 24.5%, alpha-muurolene with 19.3%, cf. EC 4.2.3.125, delta-cadinol with 18.6%, delta-elemene with 16.0%, tau-muurolene with 10.8%, and beta-elemene with 10.8% ?
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?

Organism

Organism UniProt Comment Textmining
Magnolia grandiflora B3TPQ6 gene Mg25
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Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate = beta-cubebene + diphosphate reaction mechanism, overview Magnolia grandiflora

Source Tissue

Source Tissue Comment Organism Textmining
leaf Mg25 is much more evident in young developing leaf tissues than in older, more mature leaves Magnolia grandiflora
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additional information Mg25 transcript levels are low or below detection limits in tepal and carpel tissues Magnolia grandiflora
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stamen high expression level Magnolia grandiflora
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
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Magnolia grandiflora beta-cubebene + diphosphate
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?
(2E,6E)-farnesyl diphosphate Mg25 converts farnesyl diphosphate predominantly to beta-cubebene Magnolia grandiflora beta-cubebene + diphosphate
-
?
additional information the dominant reaction products generated by Mg25 are beta-cubebene with 24.5%, alpha-muurolene with 19.3%, cf. EC 4.2.3.125, delta-cadinol with 18.6%, delta-elemene with 16.0%, tau-muurolene with 10.8%, and beta-elemene with 10.8% Magnolia grandiflora ?
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?

Subunits

Subunits Comment Organism
More the enzyme contains the conserved Asp-rich domain, DDXXD, that coordinates substrate binding via the formation of divalent cation salt bridges Magnolia grandiflora

Synonyms

Synonyms Comment Organism
Mg25
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Magnolia grandiflora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24 26
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Magnolia grandiflora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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maximal activity at pH 7.5, dominated by four products: beta-cubebene, alpha-muurolene, delta-cadinol, and tau-muurolene Magnolia grandiflora

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 no activity below or above Magnolia grandiflora

General Information

General Information Comment Organism
evolution the terpene synthase Mg25 belongs to the angiosperm sesquiterpene synthase subclass TPS-a. The structural diversity observed in the three Magnolia terpene synthase genes have occurred either by a rapid loss of introns from a common ancestor TPS gene or by a gain of introns into an intron-deficient progenote TPS gene Magnolia grandiflora
additional information two terpenes dominate the plant sesquiterpene profile, with beta-cubebene accounting for 31.3% of the total and beta-elemene for 25.3%. The enzyme contains the conserved Asp-rich domain, DDXXD, that coordinates substrate binding via the formation of divalent cation salt bridges Magnolia grandiflora