Cloned (Comment) | Organism |
---|---|
gene Mg25, DNA and amino acid sequence determination and analysis, Mg25 has one single intron located near the 5' terminus of the gene, expression analysis and phylogenetic analysis | Magnolia grandiflora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, maximal activities at Mg2+ concentrations of 1 to 10 mM | Magnolia grandiflora | |
Mn2+ | activates, naximally at concentrations of 1 mM or less | Magnolia grandiflora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Magnolia grandiflora | Mg25 converts farnesyl diphosphate predominantly to beta-cubebene | beta-cubebene + diphosphate | - |
? | |
additional information | Magnolia grandiflora | the dominant reaction products generated by Mg25 are beta-cubebene with 24.5%, alpha-muurolene with 19.3%, cf. EC 4.2.3.125, delta-cadinol with 18.6%, delta-elemene with 16.0%, tau-muurolene with 10.8%, and beta-elemene with 10.8% | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Magnolia grandiflora | B3TPQ6 | gene Mg25 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate = beta-cubebene + diphosphate | reaction mechanism, overview | Magnolia grandiflora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | Mg25 is much more evident in young developing leaf tissues than in older, more mature leaves | Magnolia grandiflora | - |
additional information | Mg25 transcript levels are low or below detection limits in tepal and carpel tissues | Magnolia grandiflora | - |
stamen | high expression level | Magnolia grandiflora | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Magnolia grandiflora | beta-cubebene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | Mg25 converts farnesyl diphosphate predominantly to beta-cubebene | Magnolia grandiflora | beta-cubebene + diphosphate | - |
? | |
additional information | the dominant reaction products generated by Mg25 are beta-cubebene with 24.5%, alpha-muurolene with 19.3%, cf. EC 4.2.3.125, delta-cadinol with 18.6%, delta-elemene with 16.0%, tau-muurolene with 10.8%, and beta-elemene with 10.8% | Magnolia grandiflora | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains the conserved Asp-rich domain, DDXXD, that coordinates substrate binding via the formation of divalent cation salt bridges | Magnolia grandiflora |
Synonyms | Comment | Organism |
---|---|---|
Mg25 | - |
Magnolia grandiflora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 26 | - |
Magnolia grandiflora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
maximal activity at pH 7.5, dominated by four products: beta-cubebene, alpha-muurolene, delta-cadinol, and tau-muurolene | Magnolia grandiflora |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | no activity below or above | Magnolia grandiflora |
General Information | Comment | Organism |
---|---|---|
evolution | the terpene synthase Mg25 belongs to the angiosperm sesquiterpene synthase subclass TPS-a. The structural diversity observed in the three Magnolia terpene synthase genes have occurred either by a rapid loss of introns from a common ancestor TPS gene or by a gain of introns into an intron-deficient progenote TPS gene | Magnolia grandiflora |
additional information | two terpenes dominate the plant sesquiterpene profile, with beta-cubebene accounting for 31.3% of the total and beta-elemene for 25.3%. The enzyme contains the conserved Asp-rich domain, DDXXD, that coordinates substrate binding via the formation of divalent cation salt bridges | Magnolia grandiflora |