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Literature summary for 4.2.3.127 extracted from

  • Jin, J.; Kim, M.J.; Dhandapani, S.; Tjhang, J.G.; Yin, J.L.; Wong, L.; Sarojam, R.; Chua, N.H.; Jang, I.C.
    The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase (2015), J. Exp. Bot., 66, 3959-3975 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene TPS2, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, transient and functional recombinant expression of YFP-tagged enzyme CoTPS2 in transgenic Nicotiana benthamiana leaves in cytosol under control of CaMV 35S promoter via Agrobacterium tumefaciens-mediated transfection method Cananga odorata var. fruticosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cananga odorata var. fruticosa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, enzyme CoTPS2 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain Cananga odorata var. fruticosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Cananga odorata var. fruticosa
-
beta-copaene + diphosphate
-
?
additional information Cananga odorata var. fruticosa enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene ?
-
?

Organism

Organism UniProt Comment Textmining
Cananga odorata var. fruticosa
-
dwarf ylang ylang, grown in Singapore
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cananga odorata var. fruticosa

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Cananga odorata var. fruticosa
-
flower bud CoTPS2 is highly expressed at the floral bud stage Cananga odorata var. fruticosa
-
leaf very low expression level Cananga odorata var. fruticosa
-
additional information the enzyme expression increases from undeveloped small flowers to mature green flowers, is absent in mature yellow flowers, but very high in flower buds Cananga odorata var. fruticosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Cananga odorata var. fruticosa beta-copaene + diphosphate
-
?
additional information enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene Cananga odorata var. fruticosa ?
-
?

Synonyms

Synonyms Comment Organism
CoTPS2
-
Cananga odorata var. fruticosa
TPS2
-
Cananga odorata var. fruticosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cananga odorata var. fruticosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Cananga odorata var. fruticosa

General Information

General Information Comment Organism
evolution the enzyme belongs to the terpene sythase superfamily, subfamily TPS-a. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS2. CoTPS2 does not contain a plastid Tp sequence Cananga odorata var. fruticosa
metabolism the enzyme is part of the biosynthesis of volatile organic compounds in flowers Cananga odorata var. fruticosa
physiological function recombinant CoTPS2 catalyses the synthesis of three compounds, beta-ylangene, beta-copaene, and beta-cubebene, from farnesyl diphosphate. CoTPS2 is a multifunctional beta-ylangene/beta-copaene/beta-cubebene synthase capable of producing three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene. The enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS2 is a multifunctional terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene Cananga odorata var. fruticosa