Cloned (Comment) | Organism |
---|---|
gene TPS2, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, transient and functional recombinant expression of YFP-tagged enzyme CoTPS2 in transgenic Nicotiana benthamiana leaves in cytosol under control of CaMV 35S promoter via Agrobacterium tumefaciens-mediated transfection method | Cananga odorata var. fruticosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cananga odorata var. fruticosa | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, enzyme CoTPS2 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain | Cananga odorata var. fruticosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Cananga odorata var. fruticosa | - |
beta-copaene + diphosphate | - |
? | |
additional information | Cananga odorata var. fruticosa | enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cananga odorata var. fruticosa | - |
dwarf ylang ylang, grown in Singapore | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | Cananga odorata var. fruticosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Cananga odorata var. fruticosa | - |
flower bud | CoTPS2 is highly expressed at the floral bud stage | Cananga odorata var. fruticosa | - |
leaf | very low expression level | Cananga odorata var. fruticosa | - |
additional information | the enzyme expression increases from undeveloped small flowers to mature green flowers, is absent in mature yellow flowers, but very high in flower buds | Cananga odorata var. fruticosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Cananga odorata var. fruticosa | beta-copaene + diphosphate | - |
? | |
additional information | enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene | Cananga odorata var. fruticosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CoTPS2 | - |
Cananga odorata var. fruticosa |
TPS2 | - |
Cananga odorata var. fruticosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cananga odorata var. fruticosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Cananga odorata var. fruticosa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the terpene sythase superfamily, subfamily TPS-a. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS2. CoTPS2 does not contain a plastid Tp sequence | Cananga odorata var. fruticosa |
metabolism | the enzyme is part of the biosynthesis of volatile organic compounds in flowers | Cananga odorata var. fruticosa |
physiological function | recombinant CoTPS2 catalyses the synthesis of three compounds, beta-ylangene, beta-copaene, and beta-cubebene, from farnesyl diphosphate. CoTPS2 is a multifunctional beta-ylangene/beta-copaene/beta-cubebene synthase capable of producing three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene. The enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS2 is a multifunctional terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene | Cananga odorata var. fruticosa |