Cloned (Comment) | Organism |
---|---|
gene brtC, functional recombinant expression in Bacillus subtilis strain BSDOI-2, and functional recombinant expression from codo-optimzed gene in Streptomyces tenebrarius | Niallia circulans |
gene tbmA, functional recombinant expression codon-optimized gene in Bacillus subtilis strain 168-DOI-15, in which both genes pgi and pgcA are disrupted | Streptoalloteichus tenebrarius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of a natural btrC gene, encoding DOI synthase in butirosin-producing Bacillus circulans, in the heterologous host Bacillus subtilis strain 168-BSDOI-2 generates approximately 2.3 g/l 2-deoxy-scyllo-inosose (DOI), fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production | Niallia circulans |
additional information | construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of gene btrC encoding DOI synthase derived from tobramycin-producing Streptomyces tenebrarius in recombinant of Bacillus subtilis strain BSDOI-15, in which both genes pgi and pgcA are disrupted, significantly enhances the 2-deoxy-scyllo-inosose (DOI) titer to up to 37.2 g/l, fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production | Streptoalloteichus tenebrarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Niallia circulans | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Streptoalloteichus tenebrarius | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Streptoalloteichus tenebrarius ATCC 17920 | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q9S5E2 | - |
- |
Streptoalloteichus tenebrarius | Q2MF16 | - |
- |
Streptoalloteichus tenebrarius ATCC 17920 | Q2MF16 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | - |
Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | - |
Streptoalloteichus tenebrarius | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | - |
Streptoalloteichus tenebrarius ATCC 17920 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
btrC | - |
Niallia circulans |
DOI synthase | - |
Niallia circulans |
DOI synthase | - |
Streptoalloteichus tenebrarius |
tbmA | - |
Streptoalloteichus tenebrarius |
General Information | Comment | Organism |
---|---|---|
physiological function | 2-deoxy-scyllo-inosose (DOI) synthase, which uses glucose-6-phosphate as a substrate for DOI biosynthesis, is indispensably involved in the initial stage of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics including butirosin, gentamicin, kanamycin, and tobramycin | Niallia circulans |
physiological function | 2-deoxy-scyllo-inosose (DOI) synthase, which uses glucose-6-phosphate as a substrate for DOI biosynthesis, is indispensably involved in the initial stage of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics including butirosin, gentamicin, kanamycin, and tobramycin | Streptoalloteichus tenebrarius |