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Literature summary for 4.2.3.124 extracted from

  • Lim, J.H.; Hwang, H.H.; Lee, N.J.; Lee, J.W.; Seo, E.G.; Son, H.B.; Kim, H.J.; Yoon, Y.J.; Park, J.W.
    Enhanced biosynthesis of 2-deoxy-scyllo-inosose in metabolically engineered Bacillus subtilis recombinants (2018), Front. Microbiol., 9, 2333 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene brtC, functional recombinant expression in Bacillus subtilis strain BSDOI-2, and functional recombinant expression from codo-optimzed gene in Streptomyces tenebrarius Niallia circulans
gene tbmA, functional recombinant expression codon-optimized gene in Bacillus subtilis strain 168-DOI-15, in which both genes pgi and pgcA are disrupted Streptoalloteichus tenebrarius

Protein Variants

Protein Variants Comment Organism
additional information construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of a natural btrC gene, encoding DOI synthase in butirosin-producing Bacillus circulans, in the heterologous host Bacillus subtilis strain 168-BSDOI-2 generates approximately 2.3 g/l 2-deoxy-scyllo-inosose (DOI), fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production Niallia circulans
additional information construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of gene btrC encoding DOI synthase derived from tobramycin-producing Streptomyces tenebrarius in recombinant of Bacillus subtilis strain BSDOI-15, in which both genes pgi and pgcA are disrupted, significantly enhances the 2-deoxy-scyllo-inosose (DOI) titer to up to 37.2 g/l, fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production Streptoalloteichus tenebrarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate Niallia circulans
-
2-deoxy-L-scyllo-inosose + phosphate
-
?
D-glucose 6-phosphate Streptoalloteichus tenebrarius
-
2-deoxy-L-scyllo-inosose + phosphate
-
?
D-glucose 6-phosphate Streptoalloteichus tenebrarius ATCC 17920
-
2-deoxy-L-scyllo-inosose + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Niallia circulans Q9S5E2
-
-
Streptoalloteichus tenebrarius Q2MF16
-
-
Streptoalloteichus tenebrarius ATCC 17920 Q2MF16
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
Niallia circulans 2-deoxy-L-scyllo-inosose + phosphate
-
?
D-glucose 6-phosphate
-
Streptoalloteichus tenebrarius 2-deoxy-L-scyllo-inosose + phosphate
-
?
D-glucose 6-phosphate
-
Streptoalloteichus tenebrarius ATCC 17920 2-deoxy-L-scyllo-inosose + phosphate
-
?

Synonyms

Synonyms Comment Organism
btrC
-
Niallia circulans
DOI synthase
-
Niallia circulans
DOI synthase
-
Streptoalloteichus tenebrarius
tbmA
-
Streptoalloteichus tenebrarius

General Information

General Information Comment Organism
physiological function 2-deoxy-scyllo-inosose (DOI) synthase, which uses glucose-6-phosphate as a substrate for DOI biosynthesis, is indispensably involved in the initial stage of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics including butirosin, gentamicin, kanamycin, and tobramycin Niallia circulans
physiological function 2-deoxy-scyllo-inosose (DOI) synthase, which uses glucose-6-phosphate as a substrate for DOI biosynthesis, is indispensably involved in the initial stage of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics including butirosin, gentamicin, kanamycin, and tobramycin Streptoalloteichus tenebrarius