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Literature summary for 4.2.3.124 extracted from
- Active site mapping of 2-deoxy-scyllo-inosose synthase, the key starter enzyme for the biosynthesis of 2-deoxystreptamine. Mechanism-based inhibition and identification of lysine-141 as the entrapped nucleophile (2004), J. Org. Chem., 69, 593-600.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene btrC, expression in Escherichia coli | Niallia circulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-carbaglucose 6-phosphate | a mechanism-based irreversible inhibitor, synthesis, overview. The alpha,beta-unsaturated intermediate traps a specific nucleophilic group in the active site through the Michael-type 1,4-addition. The covalently modified amino acid residue is Lys141 | Niallia circulans |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | required | Niallia circulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40608 | - |
x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry | Niallia circulans |
| 40768 | - |
x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry | Niallia circulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | Niallia circulans | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
| D-glucose 6-phosphate | Niallia circulans SANK72073 | - |
2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | Q9S5E2 | gene btrC | - |
| Niallia circulans SANK72073 | Q9S5E2 | gene btrC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant BtrC from Escherichia coli by gel filtration and hydrophobic interaction chromatography | Niallia circulans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate | reaction mechanism, overview | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | - |
Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
| D-glucose 6-phosphate | - |
Niallia circulans SANK72073 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40608, mass spectrometry, x * 40768, DL-carbaglucose 6-phosphate-enzyme complex, mass spectrometry | Niallia circulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| btrC | - |
Niallia circulans |
| DOIS | - |
Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
assay at | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
assay at | Niallia circulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | required | Niallia circulans | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.224 | - |
DL-carbaglucose 6-phosphate | pH 7.7, 46°C | Niallia circulans | |
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Release 2023.1 (January 2023)
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