Cloned (Comment) | Organism |
---|---|
gene btrC, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM 105, expression in Escherichia coli strain BL21(DE3) | Streptomyces fradiae |
gene btrC, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM 105, functional expression in Escherichia coli strain BL21(DE3) | Niallia circulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Niallia circulans | |
EDTA | complete inhibition | Niallia circulans | |
Ni2+ | - |
Niallia circulans | |
Zn2+ | - |
Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
D-glucose 6-phosphate | pH 7.7, 46°C, recombinant enzyme | Niallia circulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required | Streptomyces fradiae | |
Co2+ | specifically required | Niallia circulans | |
additional information | the enzyme is not affected by Mg2+, Ca2+, Mn2+, and Fe2+ | Niallia circulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 42000, BtrC, + 1 * 23000, BtrC2, recombinant enzyme, SDS-PAGE, 1 * 40746, BtrC, sequence calculation | Niallia circulans |
40746 | - |
1 * 42000, BtrC, + 1 * 23000, BtrC2, recombinant enzyme, SDS-PAGE, 1 * 40746, BtrC, sequence calculation | Niallia circulans |
42000 | - |
1 * 42000, BtrC, + 1 * 23000, BtrC2, recombinant enzyme, SDS-PAGE, 1 * 40746, BtrC, sequence calculation | Niallia circulans |
77000 | - |
gel filtration, recombinant enzyme | Niallia circulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Streptomyces fradiae | intramolecular carbocyclization, multistep process | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Niallia circulans | intramolecular carbocyclization, multistep process | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Streptomyces fradiae IFO 13147 | intramolecular carbocyclization, multistep process | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | Niallia circulans SANK72073 | intramolecular carbocyclization, multistep process | 2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q9S5E2 | gene btrC, a butirosin producing strain | - |
Niallia circulans SANK72073 | Q9S5E2 | gene btrC, a butirosin producing strain | - |
Streptomyces fradiae | - |
gene btrC, a neomycin producing strain | - |
Streptomyces fradiae IFO 13147 | - |
gene btrC, a neomycin producing strain | - |
Purification (Comment) | Organism |
---|---|
recombinant BtrC 6.0fold from Escherichia coli strain BL21(DE3) | Niallia circulans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate | reaction mechanism, overview | Streptomyces fradiae | |
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate | reaction mechanism, overview | Niallia circulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
purified recombinant BtrC, pH 7.7, 46°C | Niallia circulans |
Storage Stability | Organism |
---|---|
4°C- -75°C, purified recombinant enzyme, in 50 mM Tris-HCl buffer with 0.2 mM Co2+, loss of 60% activity within 10 days | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | intramolecular carbocyclization, multistep process | Streptomyces fradiae | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | intramolecular carbocyclization, multistep process | Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | intramolecular carbocyclization, multistep process | Streptomyces fradiae IFO 13147 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | intramolecular carbocyclization, multistep process | Niallia circulans SANK72073 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 42000, BtrC, + 1 * 23000, BtrC2, recombinant enzyme, SDS-PAGE, 1 * 40746, BtrC, sequence calculation | Niallia circulans |
Synonyms | Comment | Organism |
---|---|---|
btrC | - |
Streptomyces fradiae |
DOI synthase | - |
Streptomyces fradiae |
DOI synthase | - |
Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
assay at | Streptomyces fradiae |
55 | - |
- |
Niallia circulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, pH 7.5-8.5, rapid loss of activity above | Niallia circulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
D-glucose 6-phosphate | pH 7.7, 46°C, recombinant enzyme | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Niallia circulans |
7.7 | - |
assay at | Streptomyces fradiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required, cycling via NADH in the multistep reaction process from D-glucose 6-phosphate to 2-deoxy-L-scyllo-inosose, overview | Streptomyces fradiae | |
NAD+ | required, cycling via NADH in the multistep reaction process from D-glucose 6-phosphate to 2-deoxy-L-scyllo-inosose, overview | Niallia circulans |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of another major group of classical aminocyclitol antibiotics which contain the 2-deoxystreptamine (DOS) moiety such as neomycin, kanamycin, tobramycin, gentamicin, sisomicin, butirosin, or ribostamycin | Streptomyces fradiae |
metabolism | the enzyme is involved in the biosynthesis of another major group of classical aminocyclitol antibiotics which contain the 2-deoxystreptamine, DOS, moiety such as neomycin, kanamycin, tobramycin, gentamicin, sisomicin, butirosin, or ribostamycin | Niallia circulans |
physiological function | the DOI synthase reaction is a crucial starter step for the biosynthetic pathway of DOS-containing aminocyclitol antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI | Streptomyces fradiae |
physiological function | the DOI synthase reaction is a crucial starter step for the biosynthetic pathway of DOS-containing aminocyclitol antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI | Niallia circulans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.76 | - |
D-glucose 6-phosphate | pH 7.7, 46°C, recombinant enzyme | Niallia circulans |