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Literature summary for 4.2.3.124 extracted from
- Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans (1997), J. Antibiot., 50, 424-428.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | required | Niallia circulans |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | Niallia circulans | intramolecular carbocyclization, mechanism, overview | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
| D-glucose 6-phosphate | Niallia circulans SANK72073 | intramolecular carbocyclization, mechanism, overview | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | Q9S5E2 | gene btrC, a butirosin producing strain | - |
| Niallia circulans SANK72073 | Q9S5E2 | gene btrC, a butirosin producing strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ammonium sulfate fractionation, dialysis and two different steps of anion exchange chromatography | Niallia circulans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate | reaction mechanism, overview | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | intramolecular carbocyclization, mechanism, overview | Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
| D-glucose 6-phosphate | intramolecular carbocyclization, hydride abstraction and returning appeared to take place within the same glucose molecule | Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | product identification by GC-MS analysis | ? | |
| D-glucose 6-phosphate | intramolecular carbocyclization, mechanism, overview | Niallia circulans SANK72073 | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
| D-glucose 6-phosphate | intramolecular carbocyclization, hydride abstraction and returning appeared to take place within the same glucose molecule | Niallia circulans SANK72073 | 2-deoxy-L-scyllo-inosose + phosphate | product identification by GC-MS analysis | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DOI synthase | - |
Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Niallia circulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | essential for activity | Niallia circulans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is crucial in the carbocyclization reaction as part of the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. The DOI synthase catalyzes the intramolecular carbocyclization of D-glucose 6-phosphate into the first non-aminogenous cyclitol 2-deoxy-L-scyllo-inosose, DOI | Niallia circulans |
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Release 2023.1 (January 2023)
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