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Literature summary for 4.2.3.123 extracted from

  • Zhuang, X.; Koellner, T.G.; Zhao, N.; Li, G.; Jiang, Y.; Zhu, L.; Ma, J.; Degenhardt, J.; Chen, F.
    Dynamic evolution of herbivore-induced sesquiterpene biosynthesis in Sorghum and related grass crops (2012), Plant J., 69, 70-80.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sorghum bicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of three-dimensional structure, based on crystal structure of the 5-epi-aristolochene synthase from tobacco Sorghum bicolor

Protein Variants

Protein Variants Comment Organism
L401V products are 0.4% 7-epi-sesquithujene, 0.7% (E)-alpha-bergamotene, 1.5% sesquiabinene A, 6.2% (E)-beta-farnesene, 0% gamma-curcumene, 8.4% zingiberene, 9.5% beta-bisabolene, 73.3% beta-sesquiphellandrene Sorghum bicolor
L401V/V435S products 1.3% 7-epi-sesquithujene, 0% (E)-alpha-bergamotene, 2.1% sesquiabinene A, 17.6% (E)-beta-farnesene, 0% gamma-curcumene, 5.8% zingiberene, 30.6% beta-bisabolene, 42.6% beta-sesquiphellandrene Sorghum bicolor
L405L products are 0.9% 7-epi-sesquithujene, 1.1% (E)-alpha-bergamotene, 0.6% sesquiabinene A, 3.3% (E)-beta-farnesene, 0% gamma-curcumene, 37.5% zingiberene, 7.6% beta-bisabolene, 49.0% beta-sesquiphellandrene Sorghum bicolor
L405V products are 1.9% 7-epi-sesquithujene, 8.1% (E)-alpha-bergamotene, 8.9% sesquiabinene A, 23.9% (E)-beta-farnesene, 0% gamma-curcumene, 9.7% zingiberene, 12.0% beta-bisabolene, 26.6% beta-sesquiphellandrene, 8.9% (E)-gamma-bisabolene Oryza sativa
L405V/Y373S products are 1.2% 7-epi-sesquithujene, 23.6% (E)-alpha-bergamotene, 17.7% sesquiabinene A, 53.0% (E)-beta-farnesene, 0% gamma-curcumene, 0.6% zingiberene, 1.3% beta-bisabolene, 2.6% beta-sesquiphellandrene, 0% (E)-gamma-bisabolene Oryza sativa
V435S products are 2.2% 7-epi-sesquithujene, 0% (E)-alpha-bergamotene, 1.7% sesquiabinene A, 5% (E)-beta-farnesene, 0% gamma-curcumene, 17.8% zingiberene, 32.3% beta-bisabolene, 41% beta-sesquiphellandrene Sorghum bicolor
Y369S products are 1.1% 7-epi-sesquithujene, 0.7% (E)-alpha-bergamotene, 1.3% sesquiabinene A, 48.4% (E)-beta-farnesene, 0.4% gamma-curcumene, 44.8% zingiberene, 1.3% beta-bisabolene, 2.0% beta-sesquiphellandrene Sorghum bicolor
Y369S /V435S products are 2.5% 7-epi-sesquithujene, 2.9% (E)-alpha-bergamotene, 3.8% sesquiabinene A, 29.6% (E)-beta-farnesene, 0% gamma-curcumene, 18.6% zingiberene, 17.9% beta-bisabolene, 24.7% beta-sesquiphellandrene Sorghum bicolor
Y369S/L401V products are 2.0% 7-epi-sesquithujene, 11.4% (E)-alpha-bergamotene, 17.6% sesquiabinene A, 29.6% (E)-beta-farnesene, 4.9% gamma-curcumene, 8.2% zingiberene, 6.3% beta-bisabolene, 20% beta-sesquiphellandrene Sorghum bicolor
Y369S/L401V/V435S products are 2.0% 7-epi-sesquithujene, 6.2% (E)-alpha-bergamotene, 10.1% sesquiabinene A, 36.6% (E)-beta-farnesene, 0% gamma-curcumene, 8.2% zingiberene, 12.6% beta-bisabolene, 24.3% beta-sesquiphellandrene Sorghum bicolor
Y373S products are 3.3% 7-epi-sesquithujene, 15.6% (E)-alpha-bergamotene, 10.6% sesquiabinene A, 38.9% (E)-beta-farnesene, 0.0% gamma-curcumene, 9.8% zingiberene, 5.0% beta-bisabolene, 16.8% beta-sesquiphellandrene Sorghum bicolor
Y373S products are 7.6% 7-epi-sesquithujene, 24.8% (E)-alpha-bergamotene, 18.2% sesquiabinene A, 38.5% (E)-beta-farnesene, 0% gamma-curcumene, 4.7% zingiberene, 2.5% beta-bisabolene, 3.7% beta-sesquiphellandrene, 0% (E)-gamma-bisabolene Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Sorghum bicolor
-
beta-sesquiphellandrene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa Q0J7R9
-
-
Sorghum bicolor C5YHH7 putative
-
Sorghum bicolor C5YHI2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Sorghum bicolor beta-sesquiphellandrene + diphosphate
-
?
(2E,6E)-farnesyl diphosphate
-
Oryza sativa beta-sesquiphellandrene + diphosphate products of Oryza sativa Os08g07100 gene product are 2.5% 7-epi-sesquithujene, 5.7% (E)-alpha-bergamotene, 5.7% sesquiabinene A, 11.9% (E)-beta-farnesene, 1.5% gamma-curcumene, 30.1% zingiberene, 12.3% beta-bisabolene, 19.1% beta-sesquiphellandrene, 11.2% (E)-gamma-bisabolene ?
(2E,6E)-farnesyl diphosphate
-
Sorghum bicolor beta-sesquiphellandrene + diphosphate products of Sorghum bicolor Tps2 gene product are 1.6% (E)-alpha-bergamotene, 1.7% sesquiabinene A, 5.5% (E)-beta-farnesene, 6.7% zingiberene, 6.5% beta-bisabolene, 78.0% beta-sesquiphellandrene ?
(E,E)-farnesyl diphosphate
-
Sorghum bicolor beta-sesquiphellandrene + diphosphate products of Sorghum bicolor Tps1 gene product are 1.4% 7-epi-sesquithujene, 0.5% (E)-alpha-bergamotene, 1.0% sesquiabinene A, 2.7% (E)-beta-farnesene, 61.4% zingiberene, 5.2% beta-bisabolene, 27.8% beta-sesquiphellandrene ?
additional information in addition to using farnesyl diphosphate, Tps1 accepts geranyl diphosphate as a substrate. Myrcene, limonene and alpha-terpinolene are the major products for geranyl diphosphate substrate Sorghum bicolor ?
-
?
additional information in addition to using farnesyl diphosphate, Tps2 accepts geranyl diphosphate as a substrate. Myrcene and limonene are the major products for geranyl diphosphate substrate Sorghum bicolor ?
-
?

Synonyms

Synonyms Comment Organism
Os08g07100
-
Oryza sativa
TPS1
-
Sorghum bicolor

Expression

Organism Comment Expression
Sorghum bicolor expression is induced after caterpillar-damage to the plant up