Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sorghum bicolor |
Crystallization (Comment) | Organism |
---|---|
modeling of three-dimensional structure, based on crystal structure of the 5-epi-aristolochene synthase from tobacco | Sorghum bicolor |
Protein Variants | Comment | Organism |
---|---|---|
L401V | products are 0.4% 7-epi-sesquithujene, 0.7% (E)-alpha-bergamotene, 1.5% sesquiabinene A, 6.2% (E)-beta-farnesene, 0% gamma-curcumene, 8.4% zingiberene, 9.5% beta-bisabolene, 73.3% beta-sesquiphellandrene | Sorghum bicolor |
L401V/V435S | products 1.3% 7-epi-sesquithujene, 0% (E)-alpha-bergamotene, 2.1% sesquiabinene A, 17.6% (E)-beta-farnesene, 0% gamma-curcumene, 5.8% zingiberene, 30.6% beta-bisabolene, 42.6% beta-sesquiphellandrene | Sorghum bicolor |
L405L | products are 0.9% 7-epi-sesquithujene, 1.1% (E)-alpha-bergamotene, 0.6% sesquiabinene A, 3.3% (E)-beta-farnesene, 0% gamma-curcumene, 37.5% zingiberene, 7.6% beta-bisabolene, 49.0% beta-sesquiphellandrene | Sorghum bicolor |
L405V | products are 1.9% 7-epi-sesquithujene, 8.1% (E)-alpha-bergamotene, 8.9% sesquiabinene A, 23.9% (E)-beta-farnesene, 0% gamma-curcumene, 9.7% zingiberene, 12.0% beta-bisabolene, 26.6% beta-sesquiphellandrene, 8.9% (E)-gamma-bisabolene | Oryza sativa |
L405V/Y373S | products are 1.2% 7-epi-sesquithujene, 23.6% (E)-alpha-bergamotene, 17.7% sesquiabinene A, 53.0% (E)-beta-farnesene, 0% gamma-curcumene, 0.6% zingiberene, 1.3% beta-bisabolene, 2.6% beta-sesquiphellandrene, 0% (E)-gamma-bisabolene | Oryza sativa |
V435S | products are 2.2% 7-epi-sesquithujene, 0% (E)-alpha-bergamotene, 1.7% sesquiabinene A, 5% (E)-beta-farnesene, 0% gamma-curcumene, 17.8% zingiberene, 32.3% beta-bisabolene, 41% beta-sesquiphellandrene | Sorghum bicolor |
Y369S | products are 1.1% 7-epi-sesquithujene, 0.7% (E)-alpha-bergamotene, 1.3% sesquiabinene A, 48.4% (E)-beta-farnesene, 0.4% gamma-curcumene, 44.8% zingiberene, 1.3% beta-bisabolene, 2.0% beta-sesquiphellandrene | Sorghum bicolor |
Y369S /V435S | products are 2.5% 7-epi-sesquithujene, 2.9% (E)-alpha-bergamotene, 3.8% sesquiabinene A, 29.6% (E)-beta-farnesene, 0% gamma-curcumene, 18.6% zingiberene, 17.9% beta-bisabolene, 24.7% beta-sesquiphellandrene | Sorghum bicolor |
Y369S/L401V | products are 2.0% 7-epi-sesquithujene, 11.4% (E)-alpha-bergamotene, 17.6% sesquiabinene A, 29.6% (E)-beta-farnesene, 4.9% gamma-curcumene, 8.2% zingiberene, 6.3% beta-bisabolene, 20% beta-sesquiphellandrene | Sorghum bicolor |
Y369S/L401V/V435S | products are 2.0% 7-epi-sesquithujene, 6.2% (E)-alpha-bergamotene, 10.1% sesquiabinene A, 36.6% (E)-beta-farnesene, 0% gamma-curcumene, 8.2% zingiberene, 12.6% beta-bisabolene, 24.3% beta-sesquiphellandrene | Sorghum bicolor |
Y373S | products are 3.3% 7-epi-sesquithujene, 15.6% (E)-alpha-bergamotene, 10.6% sesquiabinene A, 38.9% (E)-beta-farnesene, 0.0% gamma-curcumene, 9.8% zingiberene, 5.0% beta-bisabolene, 16.8% beta-sesquiphellandrene | Sorghum bicolor |
Y373S | products are 7.6% 7-epi-sesquithujene, 24.8% (E)-alpha-bergamotene, 18.2% sesquiabinene A, 38.5% (E)-beta-farnesene, 0% gamma-curcumene, 4.7% zingiberene, 2.5% beta-bisabolene, 3.7% beta-sesquiphellandrene, 0% (E)-gamma-bisabolene | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Sorghum bicolor | - |
beta-sesquiphellandrene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q0J7R9 | - |
- |
Sorghum bicolor | C5YHH7 | putative | - |
Sorghum bicolor | C5YHI2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Sorghum bicolor | beta-sesquiphellandrene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | - |
Oryza sativa | beta-sesquiphellandrene + diphosphate | products of Oryza sativa Os08g07100 gene product are 2.5% 7-epi-sesquithujene, 5.7% (E)-alpha-bergamotene, 5.7% sesquiabinene A, 11.9% (E)-beta-farnesene, 1.5% gamma-curcumene, 30.1% zingiberene, 12.3% beta-bisabolene, 19.1% beta-sesquiphellandrene, 11.2% (E)-gamma-bisabolene | ? | |
(2E,6E)-farnesyl diphosphate | - |
Sorghum bicolor | beta-sesquiphellandrene + diphosphate | products of Sorghum bicolor Tps2 gene product are 1.6% (E)-alpha-bergamotene, 1.7% sesquiabinene A, 5.5% (E)-beta-farnesene, 6.7% zingiberene, 6.5% beta-bisabolene, 78.0% beta-sesquiphellandrene | ? | |
(E,E)-farnesyl diphosphate | - |
Sorghum bicolor | beta-sesquiphellandrene + diphosphate | products of Sorghum bicolor Tps1 gene product are 1.4% 7-epi-sesquithujene, 0.5% (E)-alpha-bergamotene, 1.0% sesquiabinene A, 2.7% (E)-beta-farnesene, 61.4% zingiberene, 5.2% beta-bisabolene, 27.8% beta-sesquiphellandrene | ? | |
additional information | in addition to using farnesyl diphosphate, Tps1 accepts geranyl diphosphate as a substrate. Myrcene, limonene and alpha-terpinolene are the major products for geranyl diphosphate substrate | Sorghum bicolor | ? | - |
? | |
additional information | in addition to using farnesyl diphosphate, Tps2 accepts geranyl diphosphate as a substrate. Myrcene and limonene are the major products for geranyl diphosphate substrate | Sorghum bicolor | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Os08g07100 | - |
Oryza sativa |
TPS1 | - |
Sorghum bicolor |
Organism | Comment | Expression |
---|---|---|
Sorghum bicolor | expression is induced after caterpillar-damage to the plant | up |