Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lavandula angustifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0474 | - |
geranyl diphosphate | pH 7.5, 23°C | Lavandula angustifolia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | or Mn2+, required, optimum concentration 50 mM | Lavandula angustifolia | |
Mn2+ | or Mg2+, required, optimum concentration 0.2 mM. 68% of the efficiency with Mg2+, with change in product pattern to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, for Mn2+ | Lavandula angustifolia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70300 | - |
x * 70300, calculated | Lavandula angustifolia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lavandula angustifolia | Q2XSC6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Lavandula angustifolia | (+)-alpha-pinene + diphosphate | products are 39% (R)-(+)-limonene, 22% terpinolene, 16% (1R,5S)-(+)-camphene, 14% (1R,5R)-(+)-alpha-pinene, 8% beta-myrcene and traces of alpha-phellandrene. Preferential formation of the (+)-enantiomers with 94% enantiomeric purity for (1R,5R)-(+)-alpha-pinene. Product pattern changes to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70300, calculated | Lavandula angustifolia |
Synonyms | Comment | Organism |
---|---|---|
limonene synthase | - |
Lavandula angustifolia |
LIMS | - |
Lavandula angustifolia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
optimum for total enzymic activity | Lavandula angustifolia |
33 | - |
optimum for maximum formation of alpha-pinene | Lavandula angustifolia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
geranyl diphosphate | pH 7.5, 23°C | Lavandula angustifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimum for maximum formation of alpha-pinene | Lavandula angustifolia |
7 | - |
optimum for total enzymic activity | Lavandula angustifolia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.253 | - |
geranyl diphosphate | pH 7.5, 23°C | Lavandula angustifolia |