Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | - |
Abies grandis | |
alpha-pinene | - |
Abies grandis | |
diethyldicarbonate | - |
Abies grandis | |
diphosphate | - |
Abies grandis | |
phosphate | - |
Abies grandis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
geranyl diphosphate | pH 7.8, temperature not specified in the publication | Abies grandis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | very poor substitute for Mn2+ | Abies grandis | |
Mn2+ | absolutely required, Km value 0.03 mM | Abies grandis | |
additional information | Cu2+, Cd2+, Co2+, Ni2+ and Zn2+ are ineffective | Abies grandis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
1 * 62000, SDS-PAGE | Abies grandis |
63000 | - |
gel filtration | Abies grandis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies grandis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Abies grandis |
Renatured (Comment) | Organism |
---|---|
renaturation after SDS-PAGE in 1% Tween 20 | Abies grandis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Abies grandis | - |
Storage Stability | Organism |
---|---|
purified enzyme, stable at 4°C, in 50 mM Hepes, pH 7.8, for at least 3 weeks, at -20°C, stable for several months | Abies grandis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Abies grandis | (-)-beta-pinene + diphosphate | about 40% (-)-alpha-pinene and 60% (-)-beta-pinene | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, SDS-PAGE | Abies grandis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Abies grandis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Abies grandis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | - |
80% of maximum activity | Abies grandis |
8.3 | - |
80% of maximum activity | Abies grandis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0019 | - |
pH 7.8, temperature not specified in the publication | Abies grandis | 4-hydroxymercuribenzoate | |
0.17 | - |
pH 7.8, temperature not specified in the publication | Abies grandis | diphosphate | |
0.64 | - |
pH 7.8, temperature not specified in the publication | Abies grandis | diethyldicarbonate | |
51 | - |
pH 7.8, temperature not specified in the publication | Abies grandis | phosphate |