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Literature summary for 4.2.3.120 extracted from
- Wound-inducible pinene cyclase from grand fir: Purification, characterization, and renaturation after SDS-PAGE (1992), Arch. Biochem. Biophys., 293, 167-173.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxymercuribenzoate | - |
Abies grandis |  |
| alpha-pinene | - |
Abies grandis | |
| diethyldicarbonate | - |
Abies grandis | |
| diphosphate | - |
Abies grandis | |
| phosphate | - |
Abies grandis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
geranyl diphosphate | pH 7.8, temperature not specified in the publication | Abies grandis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | very poor substitute for Mn2+ | Abies grandis | |
| Mn2+ | absolutely required, Km value 0.03 mM | Abies grandis | |
| additional information | Cu2+, Cd2+, Co2+, Ni2+ and Zn2+ are ineffective | Abies grandis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
1 * 62000, SDS-PAGE | Abies grandis |
| 63000 | - |
gel filtration | Abies grandis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Abies grandis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Abies grandis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| renaturation after SDS-PAGE in 1% Tween 20 | Abies grandis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Abies grandis | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified enzyme, stable at 4°C, in 50 mM Hepes, pH 7.8, for at least 3 weeks, at -20°C, stable for several months | Abies grandis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate | - |
Abies grandis | (-)-beta-pinene + diphosphate | about 40% (-)-alpha-pinene and 60% (-)-beta-pinene | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62000, SDS-PAGE | Abies grandis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Abies grandis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Abies grandis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
80% of maximum activity | Abies grandis |
| 8.3 | - |
80% of maximum activity | Abies grandis |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
pH 7.8, temperature not specified in the publication | Abies grandis | 4-hydroxymercuribenzoate | |
| 0.17 | - |
pH 7.8, temperature not specified in the publication | Abies grandis | diphosphate | |
| 0.64 | - |
pH 7.8, temperature not specified in the publication | Abies grandis | diethyldicarbonate | |
| 51 | - |
pH 7.8, temperature not specified in the publication | Abies grandis | phosphate | |
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