Literature summary for 4.2.3.12 extracted from

Bagautdinov, B.; Sugahara, M.; Kunishima, N.
Purification, crystallization and preliminary crystallographic analysis of archaeal 6-pyruvoyl tetrahydrobiopterin synthase homologue PH0634 from Pyrococcus horikoshii OT3 (2007), Acta Crystallogr. Sect. F, 63, 15-17.

Crystallization (Commentary)

Crystallization (Comment)	Organism
overexpressed as native and selenomethionine-substituted protein and the purified protein is crystallized by the oil-microbatch method at 22°C. 2.1 A resolution from the native crystal using synchrotron radiation at 100 K. The crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 35.83, b = 95.71, c = 05.65 A. The selenomethionine-substituted crystal is isomorphous to the native crystal and diffracts X-rays to 2.9 A	Pyrococcus horikoshii

Crystallization (Comment)

Organism

overexpressed as native and selenomethionine-substituted protein and the purified protein is crystallized by the oil-microbatch method at 22°C. 2.1 A resolution from the native crystal using synchrotron radiation at 100 K. The crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 35.83, b = 95.71, c = 05.65 A. The selenomethionine-substituted crystal is isomorphous to the native crystal and diffracts X-rays to 2.9 A

Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin	Pyrococcus horikoshii	the second of the three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin	the second of the three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP	Pyrococcus horikoshii	?	-	?

Synonyms

Synonyms	Comment	Organism
6-pyruvoyl tetrahydrobiopterin synthase	-	Pyrococcus horikoshii
PH0634	-	Pyrococcus horikoshii

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Release 2023.1 (January 2023)