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Literature summary for 4.2.3.12 extracted from
- Escherichia coli 6-pyruvoyltetrahydropterin synthase ortholog encoded by ygcM has a new catalytic activity for conversion of sepiapterin to 7,8-dihydropterin (2002), FEBS Lett., 523, 234-238.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1-5 mM, 110-120% activation | Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
| Co2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
| Cu2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
| EDTA | above 10 mM | Escherichia coli | |
| EDTA | - |
Homo sapiens | |
| Mg2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
| Mn2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
| Ni2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
| Zn2+ | 10 mM, 40-100% inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.92 | - |
sepiapterin | pH 7.5, 37ºC | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion required | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | Escherichia coli | the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | Homo sapiens | the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Synechocystis sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extraction, chromatography on a column of Ni-NTA gel | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00023 | - |
SSCR activity | Homo sapiens |
| 0.00034 | - |
SSCR activity | Drosophila melanogaster |
| 0.00039 | - |
PTPS activity | Synechocystis sp. |
| 0.00113 | - |
PTPS activity | Escherichia coli |
| 0.00891 | - |
PTPS activity | Drosophila melanogaster |
| 0.01303 | - |
PTPS activity | Homo sapiens |
| 0.09767 | - |
SSCR activity | Synechocystis sp. |
| 0.09936 | - |
SSCR activity | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70ºC, stable | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway | Escherichia coli | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway | Homo sapiens | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| sepiapterin | the enzyme cleaves the C6 side chain of sepiapterin, sepiapterin side chain releasing activity, SSCR activity | Escherichia coli | 7,8-dihydropterin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | 3.2fold increase in activity compared to that at 37°C | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Homo sapiens |
| 80 | - |
24% retention of activity after 10 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor required | Escherichia coli |
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