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Literature summary for 4.2.3.12 extracted from
- Purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from Drosophila melanogaster (1990), Biochim. Biophys. Acta, 1038, 186-194.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Drosophila melanogaster |  |
| 0.1 | - |
7,8-dihydroneopterin triphosphate | pH 7.5, 37ºC | Drosophila melanogaster | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37500 | - |
x * 37500, SDS-PAGE | Drosophila melanogaster |
| 37500 | - |
2 * 37500, also evidence for higher multimeric forms, SDS-PAGE | Drosophila melanogaster |
| 83000 | - |
gel filtration of active enzyme seems to have larger molecular mass forms | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin triphosphate | Drosophila melanogaster | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| additional information | Drosophila melanogaster | the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Affigel Blue chromatography, DEAE-Bio-gel chromatography, Affigel Blue chromatography, SDS-PAGE | Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| head | - |
Drosophila melanogaster | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0632 | - |
- |
Drosophila melanogaster |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, PIPES 50 mM, stable for 1 year | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin triphosphate | - |
Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 7,8-dihydroneopterin triphosphate | assay at | Drosophila melanogaster | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| additional information | the sequence of reaction steps include redox transfer between atoms N5, C6 and C1' and unusual triphosphate elimination at the C2'-C3' bond in the side chain | Drosophila melanogaster | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37500, SDS-PAGE | Drosophila melanogaster |
| dimer | 2 * 37500, also evidence for higher multimeric forms, SDS-PAGE | Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Drosophila melanogaster |
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