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Literature summary for 4.2.3.12 extracted from

  • Hasler, T.; Curtius, H.C.
    Purification and characterization of 6-pyruvoyl tetrahydropterin synthase from salmon liver (1989), Eur. J. Biochem., 180, 205-211.
    View publication on PubMed
Search for more literature for 4.2.3.12

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol increased catalytic activity with 10 mM Salmo salar

Inhibitors

Inhibitors Comment Organism Structure
N-(7-Dimethyl-amino-4-methyl coumarinyl) maleimide
-
 Salmo salar

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Salmo salar
0.0022
-
 7,8-dihydroneopterin triphosphate pH 7.4, 37ºC Salmo salar

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 x * 16000, SDS-PAGE Salmo salar
17000
-
 x * 17000, SDS-PAGE Salmo salar
68000
-
 gel filtration Salmo salar

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Salmo salar one of the enzymes involved in tetrahydrobiopterin biosynthesis 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
7,8-dihydroneopterin triphosphate Salmo salar assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Salmo salar
-
 salmon
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information free of mannose and glucose residues Salmo salar
no glycoprotein no carbohydrates bound Salmo salar

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydrophobic interaction chromatography, gel filtration, ion exchange, hydroxyapatite chromatography Salmo salar

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Salmo salar
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
-
 Salmo salar

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Salmo salar 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin one of the enzymes involved in tetrahydrobiopterin biosynthesis Salmo salar 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
7,8-dihydroneopterin triphosphate
-
 Salmo salar 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
7,8-dihydroneopterin triphosphate assay at Salmo salar 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 17000, SDS-PAGE Salmo salar
? x * 16000, SDS-PAGE Salmo salar

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 5 min, 25% loss of activity Salmo salar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Salmo salar
7.5
-
 in Tris-HCl 100 mM Salmo salar