Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | increased catalytic activity with 10 mM | Salmo salar |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(7-Dimethyl-amino-4-methyl coumarinyl) maleimide | - |
Salmo salar |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Salmo salar | |
0.0022 | - |
7,8-dihydroneopterin triphosphate | pH 7.4, 37ºC | Salmo salar |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
x * 16000, SDS-PAGE | Salmo salar |
17000 | - |
x * 17000, SDS-PAGE | Salmo salar |
68000 | - |
gel filtration | Salmo salar |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | Salmo salar | one of the enzymes involved in tetrahydrobiopterin biosynthesis | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | Salmo salar | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmo salar | - |
salmon | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | free of mannose and glucose residues | Salmo salar |
no glycoprotein | no carbohydrates bound | Salmo salar |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, hydrophobic interaction chromatography, gel filtration, ion exchange, hydroxyapatite chromatography | Salmo salar |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Salmo salar | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.6 | - |
- |
Salmo salar |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Salmo salar | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | one of the enzymes involved in tetrahydrobiopterin biosynthesis | Salmo salar | 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | - |
Salmo salar | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
7,8-dihydroneopterin triphosphate | assay at | Salmo salar | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16000, SDS-PAGE | Salmo salar |
? | x * 17000, SDS-PAGE | Salmo salar |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
5 min, 25% loss of activity | Salmo salar |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Salmo salar |
7.5 | - |
in Tris-HCl 100 mM | Salmo salar |