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Literature summary for 4.2.3.12 extracted from

  • Takikawa, S.; Curtius, H.C.; Redweik, U.; Leimbacher, W.; Ghisla, S.
    Biosynthesis of tetrahydropterin. Purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver (1986), Eur. J. Biochem., 161, 295-302.
    View publication on PubMed
Search for more literature for 4.2.3.12

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol increased catalytic activity with 10 mM Homo sapiens
additional information NADPH is not necessary for activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA completely inactivates the enzyme by complexation of Mg2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Homo sapiens
0.01
-
 7,8-dihydroneopterin triphosphate pH 7.5, 37ºC Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 x * 19000, SDS-PAGE Homo sapiens
19000
-
 4 * 19000, SDS-PAGE Homo sapiens
83000
-
 gel filtration, seems to have 4 identical subunits Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Homo sapiens the enzyme catalyzes the first step in the conversion of 7,8-dihydroneopterin triphosphate to tetrahydrobiopterin 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
7,8-dihydroneopterin triphosphate Homo sapiens assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydroxyapatite column, gel filtration, DEAE-Fractogel 650S column, SDS-PAGE Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
0.1
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20ºC and -70ºC, Tris HCl, several months, stable Homo sapiens
-70°C, stable for several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin the enzyme catalyzes the first step in the conversion of 7,8-dihydroneopterin triphosphate to tetrahydrobiopterin Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
7,8-dihydroneopterin triphosphate
-
 Homo sapiens 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
7,8-dihydroneopterin triphosphate assay at Homo sapiens 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE Homo sapiens
tetramer 4 * 19000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens
7.5
-
 in Tris-HCl 100 mM Homo sapiens