Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.12 extracted from

  • Inoue, Y.; Kawasaki, Y.; Harada, T.; Hatekeyama, K.; Kagamiyama, H.
    Purification and cDNA cloning of rat 6-pyruvoyl-tetrahydropterin synthase (1991), J. Biol. Chem., 266, 20791-20796.
    View publication on PubMed
Search for more literature for 4.2.3.12

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol increased catalytic activity with 10 mM Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
cloned Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ammonium sulfate 50% inactivation at 400 mM Rattus norvegicus
additional information NaCl 100 mM and KCl 100 mM have no effect on activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0091
-
 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Rattus norvegicus
0.0091
-
 7,8-dihydroneopterin triphosphate pH 7.4, 37ºC Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 65% of the activity with equal concentration of Mg2+ Rattus norvegicus
Mg2+ optimum concentration is 8 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 x * 17000, SDS-PAGE Rattus norvegicus
17000
-
 4 * 17000, SDS-PAGE Rattus norvegicus
83000
-
 HPLC gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydroneopterin triphosphate Rattus norvegicus assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P27213 Wistar, cloned
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information mature protein lacks 4 N-terminal amino acids residues Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydroxyapatite column, butyl-toyopearl chromatography, gel filtration, HPLC ion exchange column Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2883
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, stable for at least 1 year Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydroneopterin triphosphate
-
 Rattus norvegicus 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
7,8-dihydroneopterin triphosphate assay at Rattus norvegicus 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 17000, SDS-PAGE Rattus norvegicus
tetramer 4 * 17000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Rattus norvegicus
7.4
-
 assay at Rattus norvegicus